iCys User Guide

May 6, 2005 iCys Research Imaging Cytometer Hardware Reference
Functional Specifications
The following specifications apply to the iCys system.
Microscope
Motorized stage
Table 2-2 describes the specifications for the microscope used with iCys.
Table 2-2: iCys Microscope Specifications:
Olympus IX-71 microscope
base
Objectives ❑ 10x magnification — 0.3 N.A.
❑ 20x magnification — 0.5 N.A.
❑ 40x magnification — 0.75 N.A
The range and resolution of the motorized stage is:
❑ Travel range:
❍ X axis = 119.3mm
❍ Y axis 116.3mm
❑ Resolution—0.5 microns
Crosshair alignment
Eye Viewing WH10x eyepieces, F/22
Video Viewing Sony CCD Video Camera, with EPIX frame
grabber card
Brightfield Illumination
Assembly
Optional EpiFluorescence
Illumination Assembly
❑ 100W precentered Halogen bulb
❑ Brightfield power source, TH4-100
❑ NA 0.55, 27mm Working Distance
condenser with 5 position manual turret
❑ Illumination Column allows 30 degree
tilting
❑ 100W Mercury Burner
❑ Mercury Burner power source
❑ Filter Slider with three filter pockets
❑ WIG, WIBA, and NV filter cubes installed
in Fluorescence Mirror Unit Cassette
Revision B iCys Research Imaging Cytometer User Guide 2-31