iCys User Guide

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Administrator Tasks Defining Custom Carriers ❍ Rooms—Click New (or Delete, if necessary) in the Rooms section to add rooms until the number of rooms in the display equals the number of rooms on the carrier. 8. Define each room in the XY tab. You need to define the parameters separately for each room. Select a room from the Rooms pull-down.The Select Room section displays. If you know the exact shape, location, and dimensions for the room, enter them as follows: ❍ Rectangle or Circle—Specify the shape of the room. ❍ For a Rectangle: ✧ Top Right Room Corner (X and Y values)—The location of the top right corner of the room relative to the X and Y values of the Dimension field (described in Step 7). ✧ Bottom Left Room Corner (X and Y values)—The location of the bottom left corner of the room relative to the X and Y values of the Dimension field. ❍ For a Circle: ✧ Center (X and Y values)—The center of this room relative to the X and Y values of the Dimension field (described in Step 7). ✧ Diameter (in millimeters)—The diameter of the room. If you do not know these values, determine them as described in “Defining a Room Using iCys” on page C-21. 9. Open the Z tab to set the Z parameters: C-18 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Administrator Tasks The Z tab allows you to specify, or determine, the appropriate start AutoFocus positions for the carrier. The parameters are: ❍ Setup—Allows you to move the stage to a well to calibrate or to the Clear position. ✧ Room—Allows you to specify which room you want to use to calibrate the Z parameter. Select the room in the Room pull-down and click Move Stage To. The stage moves to the specified room. ✧ Move Objective to Clear—Click to move the objective to the Clear position. ❍ Configurable Positions—Specifies the required values for the carrier height (the Z parameter): ✧ Base to Well Height—Specifies the distance from the base of the carrier to the bottom of the room. ✧ Bottom Thickness—Specifies the thickness of the glass or plastic through which the microscope needs to focus. If you use #1 coverglass with this carrier, the value is 0.12. For example: Revision B iCys Research Imaging Cytometer User Guide C-19
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Contents Chapter 1 Introduction Man
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Chapter 1 Introduction This manual
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Appendix A Installing iCys Research
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Appendix B Using the iNovator Toolk
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May 6, 2005 Using the iNovator Tool
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