iCys User Guide

Generating Analytical Data Creating and Changing Scattergrams
❑ Grid—If selected, overlays a grid on the display for the axis you have selected.
The number of grid lines is based on the number of tickmarks selected in the
Ticks parameter.
❑ Label—Specify a label for this axis. The name is displayed along the axis you
selected. If Use default is checked, the axis will be labeled according to its
parameter.
❑ Feature—Select the measured parameter for each axis. The features that are
available depend on the type of event. If the feature is not appropriate for the
event (for example, Parent ID for a Primary Event), it will not appear in the
Feature pulldown.
Tip: For features designated with a (Sub *), a selection will display in the
Features pulldown for each subcontoured channel. For example, if you
have both Green and Orange subcontours, you will see the following:
Area (Sub Green)
Area (Sub Orange)
Depending on the event type, the available features are:
Table 4-2: Scattergram and Histogram Feature Parameters
Feature Meaning
Area Area in square microns of each event.
Area (Sub *) For primary events if there are subcontours—The
total area, in square microns, of all sub events
associated with the primary event.
Array XPos For tissue arrays, the x position in the array
Array YPos For tissue arrays, the y position in the array
Background When using Dynamic Background, the background
fluorescence or signal nearby each event.
Circularity How close the event is to a perfect circle. The
circularity is determined by:
perimeter2 /area
Therefore, the circularity of a perfect circle is 4π
(12.566). The larger the circularity number, the less
circular it is.
Note that, due to normalization, the circularity of an
event may be less than 12.566.
Count (Sub *) For primary events if there are subcontours—A
count of the number of sub events associated with the
primary event.
4-24 iCys Research Imaging Cytometer User Guide Revision B