iCys User Guide

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iCys Cytometric Analysis Software Application Overview iCys Task Bar Defining the Scan Area for Wells ➣ To define the scan area: 1. Click the Define Scan Area icon. The Well Scan Area dialog displays: 2. Select whether you want the scan area to be a rectangle or a circle. ✧ If rectangular, you need to set the Scan field count, which defines the number of scan fields in the scan area and the size of each scan area in microns. ✧ If circular, you first need to select Circle from the Well Scan Area dialog, and then define the Radius of each scan area in microns. 3. Click OK. When you perform either a Test Scan or Scan & Save, iCys will generate the scan areas you set. Showing Expression Levels When scanning microplates, you can show the expression levels for a specific run statistic.You can use this information to help determine if your samples are generating sufficient data. Use the Toggle shading mode icon, Show legend icon, and the run statistic dropdown to display expression levels in the well map during a scan. 3-20 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 iCys Cytometric Analysis Software Application Overview ➣ To display the expression level for a run statistic: 1. Begin a Test Scan or Scan & Save run. 2. Click the Show legend ( ) icon. The color legend displays to the right of the well map: 3. Select a run statistic from the run statistic dropdown box. For any wells that have been scanned, the well color will change to show the expression level for that feature. For example: Revision B iCys Research Imaging Cytometer User Guide 3-21
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Contents Chapter 1 Introduction Man
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Chapter 1 Introduction This manual
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Appendix A Installing iCys Research
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Appendix B Using the iNovator Toolk
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iCys User Guide

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