iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
Use the Channel checkboxes to select which data channels you want to use. iCys
can acquire real data from up to five detectors, called PMT1 to PMT4, plus the
scatter channel.
Site-specific names of each PMT are shown in the Channel dialog box. These
names are site-dependent, but can, for example, include Blue, Green, Orange, or
Long Red. If your site uses fewer than 4 PMTs, the unused PMTs are greyed out
and unavailable.
Scatter does not use a filter cube. The scatter image is generated from transmitted
light passing through the sample and striking the scatter detector.
The wavelengths detected by each PMT depend on the filter cubes installed. The
standard configuration for filter cubes is:
✧ PMT1 = Blue
✧ PMT2 = Green
✧ PMT3 = Orange
✧ PMT4 = Long Red
The filter cube arrangement is site-dependent. Your site may use different filter
cubes.
If you are using multiple lasers in two passes, each selected detector will
constitute a separate data channel. For example, PMT4, with a Long Red cube
installed, will detect propidium iodide during an Argon laser pass, and
allophycocyanin (APC) during a HeNe laser pass, thus generating two channels of
data from the same PMT. In this case, you will see both a Long Red channel and a
Long Red 2 channel.
3-42 iCys Research Imaging Cytometer User Guide Revision B