iCys User Guide

May 6, 2005 Introduction
System Overview
The iCys TM Research Imaging Cytometer is a microscope-based instrument that uses
lasers and photomultiplier tube detectors to measure constituents and other properties
of cells based on multicolor fluorescence detection. The iCys instrument scans
microplates, slides, and other carriers, analyzing and recording data about each
scanned sample.
iCys Hardware
Figure 1-1 illustrates the laser and data path of the iCys Research Imaging Cytometer.
The iCys instrument, its components, and basic operation are described in detail in
Chapter 2, “iCys Research Imaging Cytometer Hardware Reference.”
Figure 1-1: iCys Laser and Data Path
Scanning mirror
Dichroic mirrors and
optical filters
Scan lens
Photomultipliers
(PMTs)
Scatter
assembly
AutoFocus/
Relay optics
Brightfield
assembly
Camera
Eyepieces
Objectives
Focus knob
The iCys Research Imaging Cytometer consists of the following major hardware
components:
❑ The optics/electronics unit creates the laser scanning beam. It contains the
fluorescence sensors and controls laser light shutters, the motorized stage, and
other functions.
❑ The microscope unit is an Olympus IX-71 microscope with a motorized stage,
brightfield lamp, and a forward scatter detector. Optionally available are the
AutoFocus mechanism, EpiFluorescence assembly, and color camera.
Revision B iCys Research Imaging Cytometer User Guide 1-3