iCys User Guide

Generating Analytical Data Contours
Green
channel
cleared
Using Inverted Scatter for Contouring
Using the Inverted Scatter channel for contouring allows you to generate precise
contours on events with little or no fluorescence. When you use the Light Loss scatter
position, events appear dark on a bright background (light loss due to absorbance and
refraction is measured). Therefore, using Inverted Scatter generates bright events with
a dark background. With appropriate settings, the bright events can be contoured.
➣ To use Scatter for contouring:
1. In the Protocol ⇒ Detectors window choose the Virtual Channel tab.
2. Create a virtual channel using Inverse Scatter. Give the channel a meaningful
name (such as ScatterInvert). For details on creating a virtual channel, see
“Creating Virtual Channels” on page 3-43.
3. In the Scatter tab of the Detectors window, select Light Loss as the Scatter
position.
4. In the Protocol ⇒ Detectors ⇒ Virtual Channel tab, check the name, e.g.
ScatterInvert, of the new virtual channel.
5. Create a new channel module using the inverse scatter virtual channel. For
additional information on channel modules see “Channel” on page 3-52.
When you run iCys, the contoured events will appear bright on a dark background, as
shown:
4-14 iCys Research Imaging Cytometer User Guide Revision B