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May 6, 2005 <strong>iCys</strong> Cytometric Analysis Software Application Overview<br />
If you are not satisfied with the result, open the Image Registration window and click<br />
the Reset button to clear the transformation data. You can then reselect points.<br />
If you are satisfied with result, close the Merge Preview window.<br />
9. Click OK on the Merge Run window to start the merge, a message, “Merging<br />
files, please wait” displays.<br />
When the merge is complete:<br />
❑ If you selected Open Merged Run and there were changes to the current Run<br />
Workspace:<br />
❑ The Save Workspace window displays. Click the appropriate button, as described<br />
in “Saving Workspaces” on page 3-87.<br />
❑ When the merge is finished, a new Run opens that contains only the merged data.<br />
❑ If you did not select Open Merged Run, the new Run is saved, but the original<br />
Workspace and Run remains open.<br />
10. In the new Run, only the field image data and the Workspace are saved. After the<br />
Runs have been merged, you will need to perform a Scan & Save using the new<br />
merged data to generate new analytical data for the merged Run.<br />
Working With a Merged Run<br />
Reanalyzing Runs<br />
❑ After you generate new data, the merged channels are very similar to any other<br />
virtual channels (for more information about virtual channels, see “Working With<br />
Virtual Channels” on page 3-47).<br />
❑ Merged channels are available in the merged run for analysis and for creating new<br />
virtual channels.<br />
❑ Any virtual channels from the first run are brought forward (along with the other<br />
Workspace settings of the first run) into the merged run's Workspace.<br />
❑ Virtual channels, and other Workspace information, from the second Run are not<br />
brought forward into the new merged Run.<br />
❑ When using Dynamic Background, channels merged in from the second run will<br />
be background-corrected by default.<br />
There are two modes available for reanalyzing saved Runs: Analyze Events and<br />
Reanalyze. These modes are selectable in the Open Run dialogue window from the<br />
Task bar.<br />
Revision B <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> 3-97
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