iCys User Guide

May 6, 2005 iCys Research Imaging Cytometer Hardware Reference
❑ Turning on the brightfield source and viewing the specimen area directly through
the eyepiece.
❑ Using the CCD camera with either the stand alone monitor or the computer
monitor.
➣ In either case, to manually focus the microscope:
1. Lower the objective lens turret to the full downward position.
2. In the Scan Area Setup window, described in “Setting up a Microplate Scan” on
page 3-17 and “Setting up a Freeform Carrier Scan” on page 3-22, select Move
stage to active well ( ), then click on the region of interest to move the stage
into position.
3. Select Instrument ⇒ Brightfield, and switch the microscope Light Path Selector
lever to Observation ( ).
4. If necessary, adjust the brightfield lamp power.
5. From the microscope, rotate the coarse or fine focus knob until the specimen
appears to be in focus on the eyepieces, the camera monitor or the computer
monitor
6. Adjust the focus by the system offset for the objective lens used in the run.
7. The system is now ready to scan the well or scan area.
Objective Lens Automated Movement
When scanning, the microscope automatically moves the objective lens into position
for scanning. When not scanning, there are three possible locations for the objective
lens:
❑ Objective Home—The home base for the lens. The lens is entirely retracted. The
objective moves to the home position when the objective lens is explicitly
returned home. After the objective lens is homed, it is driven to the Clear Position.
❑ Clear position—The lens is retracted enough to be clear of any carriers, but is not
retracted to the Home position. The objective moves to Clear when a Scan & Save
run is completed, is manually stopped, or when the stage is moved to the Load
position.
❑ Retraction offset—A carrier-specific retraction that allows the stage to move
without bumping into the objective. The objective moves to the retraction offset
when:
❍ A Test Scan completes or is stopped.
❍ The stage is moved using the Move to Active Well command between wells
or rooms in the Scan Area Setup window.
❍ You view events in the Field Image window as described in “Locating and
Viewing Events in the Field Image Window” on page 4-45.
Revision B iCys Research Imaging Cytometer User Guide 2-9