iCys User Guide

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iCys Research Imaging Cytometer Hardware Reference Microscope Unit Brightfield Lamp The brightfield illumination column and lamp allow you to view your samples directly from the microscope stage. Brightfield is only useful when using direct observation. Figure 2-6: Brightfield Illumination Assembly When using the brightfield lamp, you also need the TH4-100 power supply that powers the lamp. For information about using the brightfield lamp, see “Observing Samples with the Brightfield Source” on page 2-14. For detailed information about the IX71 microscope and brightfield illumination, see the Olympus Instructions: IX71/IX51 Inverted Research Microscope/Inverted Basic Microscope. Forward Scatter Detector Brightfield Shutter Brightfield Filter Housing Brightfield Lamp Brightfield Power Source Light Intensity Control Knob Power Switch The Forward Scatter detector allows you to generate scatter images in iCys. You can use scatter detection to show relief details in an otherwise flat image. iCys provides two types of scatter imaging: Light Loss and Shaded Relief. Scatter detection is only available when the Scatter channel is selected in the Instrument Setup window. For information about using the Scatter detector, see “Selecting the Forward Scatter Method” on page 3-48”. 2-10 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 iCys Research Imaging Cytometer Hardware Reference Figure 2-7: Forward Scatter Assembly EpiFluorescence System Note: The iCys EpiFluorescence system is an optional feature of the Olympus IX71 microscope that allows you to view samples directly using reflected fluorescent light to highlight particular features. EpiFluorescent mirror cassette For information about using the EpiFluorescent system, see “Observing Samples using EpiFluorescence” on page 2-15. For information about using the EpiFluorescent system hardware, see “Optional EpiFluorescence Illumination Assembly” on page 2-31. For detailed information about the EpiFluorescence system, see the Olympus Instructions: Manual/Motorized Reflected Fluorescence System. Revision B iCys Research Imaging Cytometer User Guide 2-11
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Page 3 and 4: Contents Chapter 1 Introduction Man
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Appendix A Installing iCys Research
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iCys User Guide

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