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iCys User Guide

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May 6, 2005 Generating Analytical Data<br />

Using the Graph Property Window<br />

➣ To adjust the histogram, right-click on the histogram to open the Graph Property<br />

window. The Graph Property window contains three tabs:<br />

❑ Axis Tab<br />

❑ Event Type Tab<br />

❑ General Tab<br />

This section discusses the tabs in the Graph Property window.<br />

Histogram Axis Tab<br />

The Histogram Axis Tab allows to you define the X-Axis.The Histogram Axis Tab is<br />

exactly like the Scattergram Axis Tab with the exception that the Y-Axis is always<br />

Count and cannot be changed. For information about setting the Histogram Axis Tab,<br />

see “Scattergram and Histogram Axis Tab” on page 4-23.<br />

Histogram Event Type Tab<br />

This tab allows you to specify the events that will trigger a spot on the histogram.<br />

The Histogram Event Type Tab is exactly like the Scattergram Event Type Tab. For<br />

information about setting the Histogram Event Type Tab, see “Scattergram and<br />

Histogram Event Type Tab” on page 4-26.<br />

Histogram General Tab<br />

The General tab allows you to set a number of miscellaneous parameters. The<br />

parameters available from this tab are:<br />

❑ Title—Enter a title for this graph. The title appears at the top of the graph.<br />

❑ # of channels displayed—Specify the resolution of the X-axis. You can specify<br />

either 200 or 500 channels. A “channel,” in this instance, is the number of<br />

divisions along the X-axis. The default is 200.<br />

When you specify 500, <strong>iCys</strong> displays the histogram in wide mode, as follows:<br />

❑ Bin count—Specify the width of each datapoint in the bar graph. By default, the<br />

number of bins is equal to the number of channels, and each datapoint is one pixel<br />

Revision B <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> 4-39

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