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iCys User Guide

iCys User Guide

iCys User Guide

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Generating Analytical Data Creating and Changing Histograms<br />

The zoom histogram is a standard histogram. You can create regions, zoom, and make<br />

any other changes to it.<br />

Deleting a Region<br />

To delete a region from a histogram, click Delete Region.<br />

Viewing Cell Details<br />

As with scattergrams, click View Cells in Region to display the View Events<br />

window. For information about the View Events window, see “Viewing Cell Details”<br />

on page 4-35.<br />

Locating events is described in detail in “Locating and Viewing Events in the Field<br />

Image Window” on page 4-45.<br />

Other Histogram Information<br />

In addition to creating and manipulating regions, you can use the Histogram menu to:<br />

❑ Clone—If you display the Histogram menu when you do not have the cursor<br />

within a region, <strong>iCys</strong> will create a histogram that is an exact copy of the selected<br />

histogram.<br />

❑ Clone For Rx—If you display the Histogram menu when you do have the cursor<br />

within a region, <strong>iCys</strong> will create a histogram that contains only the data from the<br />

selected region and is gated to the selected region, as discussed in “Scattergram<br />

and Histogram Event Type Tab” on page 4-26.<br />

❑ Normalize XY—For XY graphs only—Tracks the X coordinates for each well.<br />

❑ Copy—Creates a snapshot image of the selected histogram (in bitmap format) and<br />

saves it to the clipboard. You can then paste the image to another application.<br />

4-44 <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> Revision B

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