iCys User Guide

Generating Analytical Data Contours
Background Contours
Background contours are available from the Protocol ⇒ Event ⇒ Background menu,
shown below.
Background contour lines are used to capture an approximate value that represents the
background fluorescence surrounding an event. The value of the background is
calculated by taking the pixels between the Use Pixels Between boundaries, and
determining a mean background value. This background value is then subtracted from
each event pixel.
In the following example, the background annulus of Event1 overlaps Event2, which
will skew the Event1 background higher. By setting Use Pixels Between, you can
limit the effect of these kinds of non-background anomalies.
Event 1
Event 2
Generating Background Contours
Determine whether you want background contours.
Event 1 background
contour lines
4-8 iCys Research Imaging Cytometer User Guide Revision B