iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
Scan and Save
Make sure that none of the dimmest pixels are reading 0 and the background pixel
values are consistently in the range of 300 to 500.
Note: Offset range is between -5 and 5 volts. Changing offset to correctly adjust
background pixels will also affect bright pixels. Check bright pixels after
offset adjustments, and adjust voltage if necessary.
Note: Staining often varies across the specimen; review multiple scan fields to
ensure that settings are appropriate.
9. When you are satisfied with the settings, you can confirm that they are optimized
by using the bright contrast tool in the field image window.
Once you are satisfied with the settings for your scan, you can click the Scan and Save
button. Scan and Save begins the formal scan process.
➣ To Start a Scan and Save:
1. Click Scan and Save from the Task Bar. The Scan and Save dialog displays:
2. Enter a Run name.
3. Enter a Run Annotation if desired
3-84 iCys Research Imaging Cytometer User Guide Revision B