iCys User Guide

May 6, 2005 iCys Research Imaging Cytometer Hardware Reference
Figure 2-10: Light Path Selector Knob
6. Focus the sample using the microscope focus knob or, if available, the rocker
switch and the fine focus knob on the Remote Control unit.
7. After the hardware setup is complete, you can use the iCys application to view the
samples on the microscope stage.
Observing Samples using EpiFluorescence
➣ If your iCys system includes the optional EpiFluorescence feature, you can use it to
observe samples as follows:
1. Set the light path selector lever to observation ( ).
2. If necessary, deselect Brightfield from the iCys Instrument menu.
3. Turn on the Power Supply Unit for the mercury burner.
4. Set the EpiFluorescence cassette to the desired filter position. The following
filters are supplied with iCys:
Table 2-1: EpiFluorescence Filters
Turret
Position
Filter Name Filter Short
Name
Eyepieces only
Eyepieces and Camera
(80% binoculars, 20% Camera)
Excitation
Light Color
5. Make sure the shutter on the EpiFluorescence cassette is open:
Camera only
2 U-MNU2 NU Violet Blue
3 UMWIBA2 WIBA Blue Green
4 U-MWIG2 WIG Green Red
Shutter in Open
Position
Emission Color
Revision B iCys Research Imaging Cytometer User Guide 2-15