iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
The following table summarizes the options in the feature field:
Table 3-4: Feature Options
Parameters Meaning
Area Area in square microns of each event.
Background Background fluorescence or signal nearby each event.
Circularity How close the event is to a perfect circle. The circularity is
defined by: perimeter2 /area
Therefore, the circularity of a perfect circle is 4π (12.566).
The larger the circularity number, the less circular it is.
Note that, due to normalization, the circularity of an event
may be less than 12.566.
Id The Id number of the event. Each event is individually tagged
with an Id number.
Integral Integrated fluorescence per event for the selected channel.
MaxPixel Brightest pixel value per event for the selected channel.
Perimeter The perimeter of the event in microns.
Scan Pixel number, from 0 to 768, indicating the y-position of the
scanning mirror where the event was detected.
Time Time, in seconds, at which the event was acquired.
When rescanning a Raw file, the Time parameter contains the
Well Number of this scan area.
WellNo Well number (or scan area number) of event.
X position X position in the carrier for each event.
Y position Y position in the carrier for each event.
3-64 iCys Research Imaging Cytometer User Guide Revision B