iCys User Guide

iCys Cytometric Analysis Software Application Overview iCys Task Bar
❍ Selecting the Forward Scatter Method
Forward Scatter allows iCys to generate images that are much different from
fluorescence images. When Scatter is selected as a channel, you can select the
Forward Scatter method you want to use:
✧ Light Loss—iCys centers the detector in the forward scattered beam.
This position emphasizes the effects of sample absorption, refraction, and
other light not picked up by the scatter detector.
✧ Shaded Relief—iCys positions the detector so the light hits the edge of
the detector. In this position, with the offset at 0, half of the forward
scattered light falls on the detector, while the other half misses the
detector. When performing a Test Scan, you can adjust the Shaded Relief
Scatter offset, described on “Setting the Shaded Relief Scatter Offset”
below.
✧ Setting the Shaded Relief Scatter Offset
If you select Shaded Relief Scatter, the Shaded Relief Scatter offset
section displays the Offset slider. Changing the Scatter offset changes the
sensor angle so that the shading is more or less prominent. Moving the
slider to the right catches more scatter signal, making the shading less
prominent; moving the beam to the left makes the shading more
prominent.
✧ To set the Shaded Relief offset:
During a Test Scan, display scatter images.
Use your mouse to move the slider left or right, then rescan and check
the image. As you move the slider, the numerical offset is shown in
the display window.
Continue making changes and rescanning until you are satisfied with
the quality of the scatter images.
3-48 iCys Research Imaging Cytometer User Guide Revision B