iCys User Guide

May 6, 2005 iCys Cytometric Analysis Software Application Overview
Selecting the Lasers and Channels
About the iCys Lasers
iCys comes with equipped with up to three lasers:
✧ Argon Ion, which emits at 488 nanometers.
✧ Helium-Neon (HeNe), which emits at 633nm.
✧ Violet, which emits at 405nm.
The lasers you select for a given scan will depend on the excitation characteristics
of the fluorophores used.
Note: Although you can select any laser or laser combination for either the first
or second pass, some laser combinations will not be compatible with the
fluorescence emission wavelengths of the dyes you are using. The
excitation wavelengths should not overlap the emission wavelengths in
which you are interested for any one pass.
Selecting Data Acquisition Channels
❍ Edit—When the Edit button on the Channel tab is selected, you can specify
the lasers and channels to be used in both the first and second passes of the
scan by clicking the Edit button. The following menu will appear.
Revision B iCys Research Imaging Cytometer User Guide 3-41