iCys User Guide

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iCys Cytometric Analysis Software Application Overview iCys Task Bar Adjusting PMT Channels Flowchart Increase Voltage% Incrementally Decrease Gain% Incrementally Decrease Offset Incrementally Scan View window: Event pixels show intensity less than 12,000 Scan View window: Event pixels intensity less than 12,000 with Voltage% > 70% Scan View window: Event pixels show intensity in range of 12,000 to 14,000 Scan View window: Pixels between events show intensity greater than 500 Scan View window: Pixels between events show intensity in range of 300 to 500 Click on Detectors Module Select Channel Tab Survey Brightest Event Survey Dimmest Background Pixels For detailed instructions on setting PMT parameters, see “Adjusting Laser Power, PMT Gains, and PMT Offsets” on page 3-82. For further information on performing a Test Scan see “Test Scan” on page 3-80. 3-40 iCys Research Imaging Cytometer User Guide Revision B Done Scan View window: Event pixels show intensity greater than 14,000 Scan View window: Pixels between events show intensity less than 300 Decrease Voltage% Incrementally Increase Offset Incrementally
May 6, 2005 iCys Cytometric Analysis Software Application Overview Selecting the Lasers and Channels About the iCys Lasers iCys comes with equipped with up to three lasers: ✧ Argon Ion, which emits at 488 nanometers. ✧ Helium-Neon (HeNe), which emits at 633nm. ✧ Violet, which emits at 405nm. The lasers you select for a given scan will depend on the excitation characteristics of the fluorophores used. Note: Although you can select any laser or laser combination for either the first or second pass, some laser combinations will not be compatible with the fluorescence emission wavelengths of the dyes you are using. The excitation wavelengths should not overlap the emission wavelengths in which you are interested for any one pass. Selecting Data Acquisition Channels ❍ Edit—When the Edit button on the Channel tab is selected, you can specify the lasers and channels to be used in both the first and second passes of the scan by clicking the Edit button. The following menu will appear. Revision B iCys Research Imaging Cytometer User Guide 3-41
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User Guide DOC190-0037-001 Revision
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Contents Chapter 1 Introduction Man
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May 6, 2005 Contents iCys Status Ba
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May 6, 2005 Contents Appendix B Usi
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Chapter 1 Introduction This manual
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Chapter 2 iCys Research Imaging Cyt
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Appendix A Installing iCys Research
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Appendix B Using the iNovator Toolk
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May 6, 2005 Using the iNovator Tool
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Appendix C Administrator Tasks This
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Appendix D Glossary This chapter co
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iCys User Guide

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