iCys User Guide

More documents

Recommendations

Info

iCys Cytometric Analysis Software Application Overview iCys Task Bar iCys Task Bar The iCys task bar is designed to guide you through the tasks necessary to set up an experiment and collect data. Tip: The task bar can be docked on either side of the screen. To change its docking location, right-click anywhere on the task bar and choose the appropriate option. The task bar consists of three main sections: the Setup area, the View area, and the Scan area. Each of these will be described in the following section. Setup Section Using the Setup section of the task bar you can: ❑ Select a Protocol Template ❑ Create a new Workspace Protocol ❑ Select a previously defined Workspace Protocol ❑ Select a Saved Run for viewing or re-analysis The tools in Setup allow you to define the settings specific to your analysis. In Setup you may define an analysis protocol or select a pre-defined protocol template. Within the protocol you may: ❍ Select lasers and PMT detectors for the experiment ❍ Select primary and secondary channels ❍ Enable phantom contouring ❍ Define or select virtual channels ❍ Set up repetitive scans ❍ Select the general instrument settings (such as carrier type, etc.) New Run iCys allows you to choose among several run templates. These templates are provided with iCys and are designed to address some of the more common types of experiments. You may use these templates as they are, or alter them to suit your needs. 3-10 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 iCys Cytometric Analysis Software Application Overview Highlighting a template displays any comments that were saved with it in the Annotation field. If a workspace is already open when you choose New Run, you will be given the option of saving changes to it before it is closed. Using the tabs you can choose from the templates provided with iCys, previously saved workspaces, or recently accessed workspaces. You can also browse for workfiles stored elsewhere. iCys Templates The following templates are included with the basic iCys software. [1] Empty The Empty template contains a 96 well plate carrier diagram and a blank protocol sheet. The Empty template can be used as a starting point in developing your own customized workspace. [2] One Primary The One Primary template has two tabs, Protocol and Data. The Data tab is an empty window provided for the display of histograms, scattergrams, etc. Revision B iCys Research Imaging Cytometer User Guide 3-11
Page 1 and 2:
User Guide DOC190-0037-001 Revision
Page 3 and 4:
Contents Chapter 1 Introduction Man
Page 5 and 6: May 6, 2005 Contents iCys Status Ba
Page 7 and 8: May 6, 2005 Contents Appendix B Usi
Page 9 and 10: Chapter 1 Introduction This manual
Page 11 and 12: May 6, 2005 Introduction System Ove
Page 13 and 14: Chapter 2 iCys Research Imaging Cyt
Page 15 and 16: May 6, 2005 iCys Research Imaging C
Page 47 and 48: Chapter 3 iCys Cytometric Analysis
Page 49 and 50: May 6, 2005 iCys Cytometric Analysi
Page 55: May 6, 2005 iCys Cytometric Analysi
Page 107 and 108:
May 6, 2005 iCys Cytometric Analysi
Page 109 and 110:
Page 111 and 112:
Page 113 and 114:
Page 115 and 116:
Page 117 and 118:
Page 119 and 120:
Page 121 and 122:
Page 123 and 124:
Page 125 and 126:
Page 127 and 128:
Page 129 and 130:
Page 131 and 132:
Page 133 and 134:
Page 135 and 136:
Page 137 and 138:
Page 139 and 140:
Page 141 and 142:
Page 143 and 144:
Page 145 and 146:
Page 147 and 148:
Chapter 4 Generating Analytical Dat
Page 149 and 150:
May 6, 2005 Generating Analytical D
Page 151 and 152:
Page 153 and 154:
Page 155 and 156:
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
Page 171 and 172:
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Page 179 and 180:
Page 181 and 182:
Page 183 and 184:
Page 185 and 186:
Page 187 and 188:
Page 189 and 190:
Page 191 and 192:
Page 193 and 194:
Page 195 and 196:
Page 197 and 198:
Appendix A Installing iCys Research
Page 199 and 200:
May 6, 2005 Installing iCys Researc
Page 201 and 202:
May 6, 2005 Installing iCys Researc
Page 203 and 204:
Appendix B Using the iNovator Toolk
Page 205 and 206:
May 6, 2005 Using the iNovator Tool
Page 207 and 208:
Page 209 and 210:
Page 211 and 212:
Page 213 and 214:
Page 215 and 216:
Page 217 and 218:
Page 219 and 220:
Page 221 and 222:
Page 223 and 224:
Page 225 and 226:
Page 227 and 228:
Page 229 and 230:
Page 231 and 232:
Page 233 and 234:
Page 235 and 236:
Page 237 and 238:
Page 239 and 240:
Page 241 and 242:
Appendix C Administrator Tasks This
Page 243 and 244:
May 6, 2005 Administrator Tasks Dis
Page 245 and 246:
May 6, 2005 Administrator Tasks Usi
Page 247 and 248:
May 6, 2005 Administrator Tasks Def
Page 249 and 250:
May 6, 2005 Administrator Tasks 5.
Page 251 and 252:
May 6, 2005 Administrator Tasks The
Page 253 and 254:
May 6, 2005 Administrator Tasks Det
Page 255 and 256:
May 6, 2005 Administrator Tasks ❍
Page 257 and 258:
Page 259 and 260:
Page 261 and 262:
May 6, 2005 Administrator Tasks ✧
Page 263 and 264:
May 6, 2005 Administrator Tasks Det
Page 265 and 266:
May 6, 2005 Administrator Tasks 5.
Page 267 and 268:
May 6, 2005 Administrator Tasks Bef
Page 269 and 270:
Appendix D Glossary This chapter co
Page 271 and 272:
May 6, 2005 Glossary C carrier The
Page 273 and 274:
May 6, 2005 Glossary EVT file An iC
Page 275 and 276:
May 6, 2005 Glossary linear amplifi
Page 277 and 278:
May 6, 2005 Glossary region A user-
Page 279 and 280:
May 6, 2005 Glossary V voltage The
show all

iCys User Guide

Create successful ePaper yourself

Delete template?

Save as template?