iCys User Guide

Generating Analytical Data Creating and Changing Histograms
wide. When you change the number of bins, the bar width of each datapoint
reflects the number of bins that will fit in each channel (that is, when both channel
number and bin count are 200, the bar width is 1 pixel wide [200/200]).
To change the number of bins, select a new bin count from the Bin count
pulldown menu. For example, in a 500 channel histogram with a bin count of 100,
the bar width for each datapoint is 5 pixels (500/100=5).
The histogram appears as follows:
❑ Plot Style—Specify whether you want the histogram to show as a bar graph or as
an outline graph. For example:
Bar Graph Outline Graph
❑ Auto Y Scale—If selected, Auto Y Scale sets the top of the Y scale to the
maximum number of counts per channel registered for this histogram during the
scan.
4-40 iCys Research Imaging Cytometer User Guide Revision B