iCys User Guide

Generating Analytical Data Creating and Changing Scattergrams
❑ Normalize when applied—If checked, automatically scales the axis for this
parameter so that all datapoints are included. The axis will be normalized only
when OK or Apply is pressed, not continuously as the data changes.
❑ Range—Allows you to specify the range of the value displayed in the graph by
entering specific values.The Range fields default to settings appropriate for the
parameter chosen for the axis.
Scattergram and Histogram Event Type Tab
This tab allows you to specify the events that will trigger a spot on a scattergram or
histogram.You can set the following parameters on the Event Type tab:
❑ Component—Select a component defined in the protocol. Events of this
component will be displayed.
❑ Gating—Specify whether you want to include all events in this graph or only
those that conform to a specific region, or “gate” on another graph. You can
combine two regions into a gate by selecting the first region, and combining it
with a second region using the Boolean operators AND, OR, or NOT.
The Gate type field indicates the type of gate specified in the first (left-hand)
field:
❍ Normal—The graph from which you are gating (that is, the current graph,
which can be either Primary or Sub) is of the same event type as the graph in
which the region(s) lie.
For normal gating, make sure that you only gate between events contoured
using the same component. If you create gates between different components,
the results are undefined.
❍ ParentToChild—The graph on which you are gating (that is, the current
graph) is a Sub event graph and the region lies within a Primary event graph.
❍ ChildToParent—The graph on which you are gating (that is, the current
graph) is a Primary event graph and the region lies within a Sub event graph.
❑ Child to Parent Mode—When the Gate type is ChildToParent, as described
above, you may have a situation as follows;
You can select one of the following options:
❍ Inclusive—Include those events that have any sub events in the region.
❍ Exclusive—Include those events that contain only the selected sub events in
the region.
In the following example:
4-26 iCys Research Imaging Cytometer User Guide Revision B