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iCys User Guide

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Glossary Glossary of Terms<br />

interquartile range, standard deviation) can be printed out in the Region Statistics<br />

window.<br />

stepper<br />

A device that controls moving the microscope stage.<br />

controller<br />

stochiometric Measurements that are proportional to the amount of substance present.<br />

Stokes shift The wavelength difference between the light absorbed by a fluorochrome and the<br />

light emitted during fluorescence.<br />

T<br />

tab A tab is a way of moving within a dialog box. (Use the TAB key.)<br />

A group of tabs along the top of a dialog box provide you with subcategories of<br />

options.<br />

taskbar In Windows XP, a taskbar is a horizontal bar located at the bottom of your screen.<br />

A button on the taskbar indicates a program that is open.<br />

In <strong>iCys</strong>, the Taskbar displays standard tasks, usually on the right-hand side of the<br />

<strong>iCys</strong> window.<br />

text box An area in a dialog box where the user can enter text.<br />

texture The measure of the variability of fluorescent intensity of a cell. Cells that have<br />

increasing pixel values from the cell’s edge to the peak have a relatively low<br />

texture value. Cells that have undulating pixel values have relatively high texture<br />

measurements.<br />

threshold An instrument setting that is used to determine a boundary based on the intensity<br />

of the photodetector signals. The threshold assists the user in making the optimum<br />

instrument settings. Once these settings are made, the measurements on those<br />

events can be saved for later analysis.<br />

.TIF file (Tagged-Image File Format) A bitmap graphics file of an image.<br />

tissue<br />

A slide that contains many small pieces, or cores, of sample tissue. Using the<br />

microarray iNovator Application Development Toolkit, you can use <strong>iCys</strong> to label and scan<br />

tissue array samples.<br />

title bar The horizontal bar at the top of a <strong>iCys</strong> window that displays a label for the<br />

contents of the window.<br />

toolbar An area on the desktop used to place icons. The icons placed in the toolbar can be<br />

customized by the user.<br />

torus An area bounded by two user-defined contours. It is used to determine the amount<br />

of fluorescence around the perimeter of a cell and also for calculating the amount<br />

of background fluorescence.<br />

2-parameter A color plot of the values of two user-selected parameters with color representing<br />

histogram<br />

either the density or an expression. 2-parameter histograms are set from the<br />

Scattergram menu in <strong>iCys</strong>.<br />

D-10 <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> Revision B

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