iCys User Guide

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Administrator Tasks Using the Light Meter Utility Using the Light Meter Utility The Light Meter utility allows you to measure the throughput of the system lasers, and determine if any action is needed to improve laser throughput. ➣ To use the Light Meter Utility, click on Light Meter. The following window displays: 1. Follow the directions in the Operator Actions window, that is, make sure that: ❍ The iCys and the lasers are warmed up. ❍ There is no carrier on the stage. ❍ For the primary Argon measurement, set the turret so no objective is selected. If necessary, unscrew the cap in the empty objective slot. Note: If your system does not have AutoFocus, you will need to manual move the objective to be level with the stage. A message will display reminding you to do so. 2. Once the instrument is correctly set up and warmed up, select Argon (488 nm.) and click the Measure or Measure Baseline button. (If no Baseline data is available, only the Measure Baseline button is available.) The measurement is recorded in the appropriate window. 3. iCys digitizes the scan line produced by the Argon laser. To save that measurement, click Save. 4. Ideally, you want the measurement value to be close to, but not at, the maximum signal intensity of 16383. To get good readings, you may need to adjust the Gain, Offset, and for the Argon measurements, the power. These settings are described in “Optimizing PMT settings” on page 3-39. C-24 iCys Research Imaging Cytometer User Guide Revision B
May 6, 2005 Administrator Tasks 5. Measure and, if desired, save, measurements for any other objectives and lasers. Note that the objectives only need to measured once (with the Argon laser). If they are dirty, then it will affect the measurements for any laser. 6. Once the measurement has been saved as the baseline, you can remeasure it at any time. The new measurement is taken against the baseline, as shown: 7. To view the current measurements, click History to display the Measurement History file: The History file can also help you track any change in measurement. If the objectives need to be cleaned, for example, their measurements will degrade. In this case, contact your CompuCyte service representative. Revision B iCys Research Imaging Cytometer User Guide C-25
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Contents Chapter 1 Introduction Man
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Chapter 1 Introduction This manual
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Appendix A Installing iCys Research
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Appendix B Using the iNovator Toolk
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