iCys User Guide

Generating Analytical Data Contours
Assigning Contouring Channels
➣ To assign contour channels:
1. From the Protocol window, choose Contour ⇒ Channel and click in the field to
the right of the Protocol menu to create a new Channel module, or click on an
existing Channel module.
Contour ⇒ Channel
The following module settings will be displayed.
2. .Select the channel, real or virtual, that you want as the contour channel from the
Channel pull-down menu.
Setting the Threshold
The Threshold represents the minimum amount of pixel fluorescence required to
generate a contour for an event.
Settings for the Threshold contour are accessible from the Protocol ⇒ Threshold
menu.
4-4 iCys Research Imaging Cytometer User Guide Revision B