iCys User Guide

May 6, 2005 iCys Research Imaging Cytometer Hardware Reference
iCys Specimen Carriers
The first step in running a scan is to load your specimens. After the specimens are
loaded, you can set up scan parameters as described in Chapter 3, “iCys Cytometric
Analysis Software Application Overview.”
iCys provides a number of options for specimen carriers:
❑ Microtiter plates (also referred to as “microplates” or “plates”) with 6, 12, 24, 48,
96 or 384 wells.
❑ Single standard slide.
❑ Four slide carriers.
❑ 3-petri dish holder.
❑ Chamber slide.
❑ Clatch slide—a type of chamber slide used for immunophenotyping studies.
Additionally, you can define custom carriers for iCys, as described in Appendix C,
“Administrator Tasks.”
About Specimen Carriers
All specimen carriers fit onto the plate holder on the iCys stage. iCys accepts the
following types of carriers:
❑ Microtiter plates. In general, microplates contain cellular or DNA samples.
CompuCyte recommends the following types of microplates:
❍ 96-well, plastic (such as Whatman cat# 7716-2380)
❍ 96-well, glass, skirtless (such as Whatman cat# 7716-2370)
❍ 96-well, glass, skirted (such as Whatman cat# 7716-2375)
❍ 48-well, tissue culture-treated (such as BD Falcon #353078)
❍ 24-well, tissue culture-treated (such as BD Falcon #353047)
❍ 12-well, tissue culture-treated (such as BD Falcon #353043)
❍ 6-well, tissue culture-treated (such as BD Falcon #353046)
❍ 384-well, optical quality plastic (such as Whatman #7706-2103)
❍ 384-well, optical quality plastic (such as Greiner #781096)
❑ Standard slide carriers and “4-slide carriers.” Slides are used for both tissue and
cellular samples.
Slide carriers can be ordered from Compucyte.
❑ Petri dish carriers. Petri dish carriers can hold up to three 35mm x 10mm culture
dishes (such as Corning #430165).
Petri dish carriers can be ordered from Compucyte.
❑ Chamber slide carriers. Chamber slide carriers are designed to hold the Lab-Tek II
2-chamber slides (Nalge Nunc #155379).
Revision B iCys Research Imaging Cytometer User Guide 2-17