iCys User Guide

Generating Analytical Data Locating and Viewing Events in the Field Image Window
If your system does not have AutoFocus, you will need to adjust the focus
by the appropriate offsets for the current action (Scan and/or
Observation).
Locating and Viewing Events using Saved Data
When you are locating events in runs that have already been saved, you can use the
previously saved data.
1. Open a run, selecting “Analyze Events.”
2. When the run is open, use Select Active Well in the Carrier window, as described
in Table 3-1, “Microplate Scan Area Setup Window,” on page 3-18 or Table 3-2,
“Freeform Scan Area Setup Window,” on page 3-24, to determine which set(s) of
data you want to activate.
3. Right-click on the region of interest and select View Cells in Region to open the
View Events window and select the event you want to view, as described in
“Viewing Cell Details” on page 4-35.
4. You can choose to view the selected event in the Field Image window, directly
with the eyepieces, with the camera, or in any combination of the three.
❍ To view the event in the Field Image window, select Scan from the View
Mode box in the View Events window.
❍ To view the event using eyepieces, camera, or both select Observation.
❍ To view the event in the Field Image window and with the eyepieces, camera,
or both, select both Scan and Observation.
5. Click on the row of the event of interest (or default to the first event), then click
View to acquire the image of the highlighted event. iCys locates the image as
follows:
❍ If Field Image is selected, the .jpg image of field that contains the event of
interest is displayed in the Field Image window. The live sample is not used
(no stage or microscope objective movement required).
❍ If both Scan and Observation are selected:
✧ The stage moves so the event of interests is in the middle of the field of
view through the eyepieces or color camera monitor.
✧ If your system has AutoFocus, iCys moves the microscope objective to
the focus position used during the original scan. If your system does not
have AutoFocus, you will need to manually focus the objective on the
event of interest.
✧ To view the event through the eyepieces or camera monitor, turn the Light
Path Selector Lever to the Observation position ( ).
❍ If Observation is selected:
4-48 iCys Research Imaging Cytometer User Guide Revision B