iCys User Guide

May 6, 2005 Generating Analytical Data
5. Click on the row of the event of interest (or default to the first event), then click
View to acquire the image of the highlighted event. iCys locates the image as
follows:
❍ If Field Image is selected:
✧ The stage moves to the start of the scan field that contains the event of
interest.
✧ iCys moves the microscope objective to the focus position used during the
original scan.
✧ The scan field is rescanned using the setup from the original scan (number
of lasers, PMTs, and so on).
✧ The event is displayed and highlighted by a red box in the Field Image
window.
❍ If both Scan and Observation are selected:
✧ After the appropriate scan field is rescanned, the stage moves so the event
of interests is in the middle of the field of view through the eyepieces or
color camera monitor.
✧ iCys moves the microscope objective to the focus position used during the
original scan.
✧ To view the event through the eyepieces or camera monitor, turn the Light
Path Selector Lever to the Observation position ( ).
❍ If Observation is selected, the event is not rescanned.The following steps
occur:
✧ The stage moves so the event of interests is in the middle of the field of
view through the eyepieces or color camera monitor.
✧ iCys moves the microscope objective to the focus position used during the
original scan.
Note: When Observation is selected, iCys will turn the Brightfield on for
viewing. Brightfield will be turned off when viewing an event with
observation unchecked, or when closing the View Events window.
In Observation-only mode, the event is not displayed in iCys.
If Observation-only is selected, make sure the Light Path Selector lever is
also in the Observation ( ) position.
Note: To locate an event for which the Time value (in the View Events window)
is different from the previous event’s value, and Scan is selected (with or
without Observation), make sure the Light Path Selector lever is set to the
Side Port position before selecting Next or View. This is required because
the newly selected event is in a different scan area and will be rescanned.
Revision B iCys Research Imaging Cytometer User Guide 4-47