iCys User Guide

Administrator Tasks Defining Custom Carriers
b. Turn the oscilloscope off by clicking the “Play or Stop” button ( ).
c. Press Set from X,Y for the Top Right Room Corner measurement.
d. Turn the oscilloscope on again, open shutter, and adjust the stage until the
bottom (front) left corner of the room is over the objective.
e. Press Set from X,Y for the Bottom Left Room Corner.
If the room is a Circle:
a. Use the touchpad buttons and Remote Control unit X and Y knobs to drive the
stage to a position such that the room is centered over the microscope beam.
b. Turn the oscilloscope off by clicking the “Play or Stop” button ( ).
c. Press Set from X,Y for the Center measurement.
d. Turn the oscilloscope on again and open shutter.
e. For the Diameter measurement, you can measure the room diameter
manually, look up the room size on the specification sheet for this carrier and
enter it for the Diameter value, or use the Remote Control unit X and Y knobs
to drive to the edge of the room and click on Set from X, Y for the Diameter
field.
8. Repeat Step 8 for each room you need to define.
Determining the Freeform Z Parameters
Finding the Base to Well Height
➣ To determine the Base to Well Height:
1. With the stage at room R1, the same objective in place as displayed in the task bar,
and the Oscilloscope window open and on, and the Argon Laser Shutter open, use
the rocker switch on the Remote Control unit to drive the objective upwards
slowly until you observe a reasonably well focused line or lines on the autofocus
monitor. Make sure that you are focusing on the first line or pair of lines to be
imaged on the display.
2. Use the Fine Focus knob on the Remote Control unit to create a well focused
image.
1. Click Set from Z for the Base To Well Height field in the Z tab. Mark down this
value, and then take the same measurement at least once each room on the carrier
(so that you have measurements for a number of positions on the carrier.)
2. Enter the lowest value measured as the Base to Well Height.
C-22 iCys Research Imaging Cytometer User Guide Revision B