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iCys User Guide

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May 6, 2005 Contents<br />

Appendix B Using the iNovator Toolkit<br />

iNovator-specific Templates ...............................................................................................B-2<br />

References to Using the Protocol Menu Bar and to Building and Modifying<br />

Protocols ........................................................................................................................B-7<br />

Using the Protocol Menu Bar ........................................................................................ B-7<br />

Building and Modifying Protocols ................................................................................ B-7<br />

iNovator-specific Protocol Modules ..................................................................................B-8<br />

Scan ................................................................................................................................ B-8<br />

Mosaic Scan Module ................................................................................................B-8<br />

Output .......................................................................................................................... B-10<br />

Processed Image .....................................................................................................B-10<br />

Image View .............................................................................................................B-11<br />

Filter Modules .............................................................................................................. B-11<br />

Image Filtering—the Filter Module ........................................................................B-11<br />

Binary Image Filtering—Erode, Dilate, Open, Close Modules ..............................B-15<br />

Watershed Module ..................................................................................................B-18<br />

Operation ..................................................................................................................... B-20<br />

Arithmetic operators ...............................................................................................B-20<br />

Boolean operators ...................................................................................................B-21<br />

Bright Contrast Module ..........................................................................................B-23<br />

Event ............................................................................................................................ B-24<br />

Scan Area Event ......................................................................................................B-24<br />

Multi-Scale Association ..........................................................................................B-26<br />

Array Alignment .....................................................................................................B-26<br />

Using the Protocol Menu View Image Feature with iNovator ......................................B-28<br />

Multi-scale Scanning .........................................................................................................B-30<br />

Introduction .................................................................................................................. B-30<br />

Unique Features ........................................................................................................... B-30<br />

Different Detector Module Settings ........................................................................B-30<br />

Different Scan Module Settings ..............................................................................B-30<br />

Scan Area Events and Other Events .......................................................................B-31<br />

Manipulating Dynamic Scan Areas ........................................................................B-31<br />

Image Hierarchy .......................................................................................................... B-34<br />

Analysis Modes for Multi-scale Scans ........................................................................ B-34<br />

New Runs ................................................................................................................B-34<br />

Reanalyzing Existing Runs .....................................................................................B-35<br />

Analyze events (bypass-bypass) .............................................................................B-36<br />

Reanalyze scale 1 (virtual-bypass) .........................................................................B-36<br />

Reanalyze scale 1 and scan scale 2 (virtual-live) ...................................................B-36<br />

Reanalyze scale 2 (bypass-virtual) .........................................................................B-36<br />

Appendix C Administrator Tasks<br />

About Administrator Tasks .............................................................................................. C-2<br />

Displaying the Administration Menu ............................................................................... C-3<br />

Opening the Administration Menu ................................................................................ C-3<br />

Changing the Password .................................................................................................. C-4<br />

Hiding the Administration Menu ................................................................................... C-4<br />

Revision B <strong>iCys</strong> Research Imaging Cytometer <strong>User</strong> <strong>Guide</strong> vii

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