QF0159 Marketing Release Record

Primary Antibodies
Human tonsil: immunohistochemical staining for retinoblastoma gene protein using NCL-RB-
358. Note intense nuclear staining of epithelial cells. Paraffin section.
Novocastra S-100
Polyclonal Reference Range
1 mL lyophilized NCL-S100p FP RUO*
1 mL liquid polyclonal NCL-L-S100p FP IVD
7 mL ready-to-use RTU-S100p FP RUO*
7 mL BOND ready-to-use PA0900 P (Enzyme)
Antigen Background
S-100A and S-100B proteins are two members of the S-100 family of proteins.
S-100A is composed of an alpha and beta chain whereas S-100B is composed
of two beta chains. S-100 protein is reported to be expressed in
neuroectodermal tissue, including nerves and melanocytes. Langerhans
cells in skin and interdigitating reticulum cells in the paracortex of lymph
nodes are also reported to express S-100 protein. It is noteworthy that S-100
protein is highly soluble and may be eluted from frozen tissue during
immunohistochemical procedures.
Product Specific Information
NCL-S100p, NCL-L-S100p and RTU-S100p may require enzyme pretreatment
in some cases and are also reactive with S-100 protein from cow, chicken,
pig, dog, cat, monkey, horse, mouse and rat.
Novocastra S100A7 (Psoriasin)
Clone 3DC
1 mL liquid NCL-L-S100-A7 P (HIER)
S100A7, also known as psoriasin, is a member of the S-100 gene family of
calcium binding proteins which regulate a variety of intracellular and
extracellular processes. It is largely, although not entirely, reported to be
confined to stratified squamous epithelium. Reports indicate that in breast
cancer, high levels of S100A7 are associated with estrogen and
progesterone receptor negative status and nodal metastasis. It is also
reported to be expressed in abnormal keratinization in squamous cell
carcinoma.
Human malignant melanoma: immunohistochemical staining for S100A7 using NCL-L-S100A7.
Note intense staining of tumor cells. Paraffin section.
/ 100
RUO*
For detailed information on all products please visit our website:
www.leica-microsystems.com
IVD
Novocastra Sarcoglycan Antibodies
Clone Ad1/20A6
1 mL, 0.1 mL lyophilized sarcoglycan, alpha (adhalin)
NCL-a-SARC FWE IVD
1 mL liquid sarcoglycan, alpha (adhalin)
NCL-L-a-SARC FWE IVD
Clone �Sarc1/5B1
1 mL, 0.1 mL lyophilized sarcoglycan, beta
NCL-b-SARC FE IVD
1 mL liquid sarcoglycan, beta NCL-L-b-SARC FE
Clone �Sarc3/12C1
1 mL lyophilized sarcoglycan, delta NCL-d-SARC FW
Clone 35DAG/21B5
1 mL, 0.1 mL lyophilized sarcoglycan, gamma
NCL-g-SARC FE IVD
Antigen Background
In normal skeletal muscle, dystrophin, the protein product of the gene which
is defective in Duchenne and Becker muscular dystrophy, is attached to the
muscle membrane via a complex of at least seven proteins (dystrophin
associated glycoproteins, DAGs). Dystrophin-deficient muscle shows a
generalized reduction in DAG labeling. Recent studies have shown that
expression of different members of the dystrophin glycoprotein complex are
altered in several types of muscular dystrophy: the picture is complex and
disease classification is currently under review. For example, individuals with
LGMD2D have mutations in the gene for alpha-sarcoglycan, those with
LGM2E have mutations in the beta-sarcoglycan gene, and those with LGM2F
have mutations in the delta-sarcoglycan gene. Also, many individuals with
severe childhood autosomal recessive muscular dystrophy (SCARMD) show
defective expression in the sarcoglycan subgroup of complex proteins which
includes alpha-sarcoglycan (adhalin) and gamma sarcoglycan. As the
sarcoglycans function together as a subcomplex, individuals with mutations
in any one of the sarcoglycan genes usually show reduced expression for the
whole group, but the reduction may be most severe for the mutated single
protein. Labeling for beta-spectrin is necessary to monitor membrane
integrity.
Western blot: detection of alpha-sarcoglycan (50 kD) using NCL-L-a-SARC. Lane A, molecular
weight markers. Lane B, human muscle extract immunoblotted with NCL-L-a-SARC.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD
IVD