QF0159 Marketing Release Record

Human glomangioma: immunohistochemical staining for CD31 antigen (PECAM-1) using
NCL-CD31-1A10. Note intense membrane staining of endothelial cells. Paraffin section.
Novocastra CD33
Clone PWS44 Reference Range
1 mL, 0.1 mL liquid NCL-L-CD33 P (HIER) W
7 mL BOND ready-to-use PA0555 P (HIER)
Antigen Background
CD33 antigen is reported to appear on myelomonocytic precursor cells after
CD34 antigen expression. It then continues to be expressed on both the
myeloid and monocyte lineages, although it is reported to be absent on
granulocytes. It has been reported that expression of CD33 is restricted to
monocytes, premyelocytes, myeloid blasts, some acute undifferentiated
leukemias and acute lymphoblastic leukemias. The expression of CD33
antigen has been demonstrated to be an important marker for distinguishing
myeloid from the lymphoid leukemias.
Acute myeloid leukemia: immunohistochemical staining for CD33 antigen using NCL-L-CD33.
Note intense cytoplasmic and membrane staining of malignant cells. Paraffin section.
Novocastra CD34 (Endothelial Cell
Marker)
Clone QBEnd/10 Reference Range
1 mL, 0.1 mL lyophilized NCL-END F P (Enzyme) C
1 mL liquid NCL-L-END F P (Enzyme) C IVD
7 mL ready-to-use RTU-END F P (Enzyme) IVD
7 mL BOND ready-to-use PA0212 P (HIER) IVD
Antigen Background
CD34 antigen is a single chain transmembrane glycoprotein with a molecular
weight of 110 kD. The CD34 protein is selectively expressed on human
lymphoid and myeloid haemapoietic progenitor cells. The CD34 protein is also
expressed on vascular endothelium.
Product Specific Information
Enzyme digestion of paraffin sections is recommended with Clone QBEnd/10
in preference to heat induced epitope retrieval as it produces stronger
staining and reduces background elastin staining.
IVD
IVD
IVD
Human tonsil: immunohistochemical staining for CD34 antigen using NCL-END. Note intense
staining of neoplastic endothelial cells and absence of staining of stromal cells. Paraffin
section.
Novocastra CD35
Clone RLB25
1 mL lyophilized NCL-CD35 ASR
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra CD37
Clone CT1
1 mL lyophilized NCL-CD37 F P (HIER)
CD37 antigen is a member of the TM4 superfamily with a molecular weight of
40 to 52 kD. CD37 antigen was originally defined as an antigen of mature
B lymphocytes where it is highly expressed. It is reported not to be
expressed on pre-B cells or plasma cells and is expressed only at low level
in T cells, neutrophils, monocytes and some myelomonocytic leukemia cells.
NK cells, platelets and erythrocytes also do not express CD37 antigen. CD37
antigen on B cells associates non-covalently with MHC class II, CD19 and
CD21 antigens and with other TM4 superfamily molecules CD53, CD81 and
CD82.
Novocastra CD38
Clone SPC32
1 mL, 0.1 mL lyophilized NCL-CD38-290 F P (HIER)
1 mL liquid NCL-L-CD38-290 F P (HIER) IVD
Antigen Background
RUO*
The CD38 molecule is a type II single transmembrane glycoprotein with a
molecular weight of 46 kD. It is an ectoenzyme with the activities of ADPribosyl
cyclase, cyclic ADP-ribose hydrolase, NAD glycohydrolase and is
involved in both the formation and hydrolysis of cADPR, a second messenger
that regulates the mobilization of intracellular Ca 2+ ions. Although the CD38
molecule was originally identified as a T lymphocyte differentiation antigen, it
is reported to be expressed in a wide range of cells and tissues. CD38 antigen
can deliver potent growth and differentiation signals to lymphoid and myeloid
cells. It is found on immature cells of the B and T cell lineages but not on most
mature resting peripheral lymphocytes. It is also present on thymocytes, pre-
B cells, germinal center B cells, mitogen-activated T cells, Ig-secreting
plasma cells, monocytes, NK cells, erythroid and myeloid progenitors in the
bone marrow and brain cells. CD38 antigen has also been reported in
neurofibrillary tangles, the pathological indicator of Alzheimer's disease that
occurs in the neuronal perikarya and proximal dendrites.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
/39
Primary Antibodies