QF0159 Marketing Release Record

Human small intestine, T cell lymphoma: immunohistochemical staining for CD2 antigen (LFA-2)
using NCL-CD2-271. Note intense membrane staining of T lymphocytes. Paraffin section.
Novocastra CD3
Clone LN10 Reference Range
1 mL, 0.1 mL liquid NCL-L-CD3-565 P (HIER)
7 mL BOND ready-to-use PA0553 P (HIER)
Clone PS1
1 mL, 0.1 mL lyophilized NCL-CD3-PS1 P (HIER) W
1 mL liquid NCL-L-CD3-PS1 P (HIER) W IVD
7 mL ready-to-use RTU-CD3-PS1 P (HIER) IVD
Clone UCHT1
1 mL lyophilized NCL-CD3 FC RUO*
Clone LN10 was developed to produce superior staining with PBS based
buffers compared to clone PS1 on paraffin sections.
Antigen Background
The CD3 molecule consists of five different polypeptide chains with molecular
weights ranging from 16 to 28 kD. The CD3 antigen is first detected in early
thymocytes and its appearance probably represents one of the earliest signs
of commitment to the T cell lineage.
Product Specific Information
Clone PS1 is specific for the non-glycosylated epsilon chain of the human CD3
molecule (Chetty R and Gatter K. Journal of Pathology. 173: 303-307 (1994)).
Clone LN10, our newest clone, is also specific for the non-glycosylated
epsilon chain of the human CD3 molecule. Clones LN10, PS1, and UCHT1
recognize T cells in thymus, bone marrow, peripheral lymphoid tissue and
blood and are all pan T cell markers.
Normal human tonsil: immunohistochemical staining for CD3 antigen using NCL-L-CD3-565.
Note intense membrane staining of T lymphocytes. Paraffin section.
IVD
IVD
IVD
Novocastra CD4
Clone 4B12
1 mL, 0.1 mL lyophilized NCL-CD4-368 F P (HIER) W
1 mL liquid NCL-L-CD4-368 ASR
7 mL BOND ready-to-use PA0368 P (HIER) IVD
Clone 1F6
1 mL, 0.1 mL lyophilized NCL-CD4-1F6 P (HIER) W IVD
1 mL liquid NCL-L-CD4-1F6 P (HIER) W IVD
7 mL ready-to-use RTU-CD4-1F6 P (HIER) IVD
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra CD5
Clone 4C7 Reference Range
1 mL, 0.1 mL lyophilized NCL-CD5-4C7 P (HIER) W
1 mL liquid NCL-L-CD5-4C7 P (HIER) W IVD
7 mL ready-to-use RTU-CD5-4C7 P (HIER) IVD
7 mL BOND ready-to-use PA0168 P (HIER) IVD
Antigen Background
CD5 antigen is reported to be expressed on 95 percent of thymocytes and 72
percent of peripheral blood lymphocytes. In lymph nodes, the main reactivity
is observed on T cells. CD5 antigen is also expressed by many T cell
leukemias, lymphomas, activated T cells and on a subset of B cells located
primarily in the mantle zones of normal lymph nodes. CD5 antigen expression
is also reported in T cell acute lymphocytic leukemias (T-ALL), some B cell
chronic lymphocytic leukemias (B-CLL) as well as B and T cell lymphomas.
Human mantle cell lymphoma: immunohistochemical staining for CD5 antigen using
NCL-CD5-4C7. Note intense membrane staining of tumor cells. Paraffin section.
RUO*
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
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Primary Antibodies