QF0159 Marketing Release Record

Human primitive neuroectodermal tumors: immunohistochemical staining for CD99 using
NCL-L-CD99-187. Paraffin section.
Novocastra CD123
Clone BR4MS
1 mL, 0.1 mL liquid NCL-L-CD123 P (HIER)
Antigen Background
The CD123 antigen is also known as the alpha subunit of the human
interleukin-3 receptor. It is a type I transmembrane glycoprotein and is a
member of the cytokine receptor superfamily. CD123 forms a heterodimer
with CD131 (the beta subunit of the interleukin-3 receptor) to form the
interleukin-3 receptor, where the cytokine specificity is provided by the alpha
subunit and the signal transduction function is provided by the beta subunit.
The interleukin-3 receptor is reported to be expressed on monocytes,
neutrophils, basophils, eosinophils, megakaryocytes, proliferation and
differentiation of these cells. Outside the hematopoietic system CD123 is
reported to be expressed in Leydig cells of the testis, some endothelial cells,
and cells of the placenta and brain.
Human high venule endothelium and plasmacytoid dendritic cells: immunohistochemical
staining for CD123 using NCL-L-CD123. Paraffin section.
Novocastra CD134 (OX40)
Clone 102H6
1 mL lyophilized NCL-CD134 F P (HIER)
The CD134 molecule is a member of the tumor necrosis factor receptor
superfamily. It was originally named MRC OX40 after the first antibody which
led to its discovery. CD134 protein binds to OX40 ligand and is expressed
specifically on activated T lymphocytes with maximum expression determined
at twenty four hours post stimulus. In rats, CD134 protein is only
expressed on activated CD4 positive T lymphocytes and in humans it is
described as being found predominantly on CD4 positive cells. In mice,
CD134 protein is expressed on both activated CD4 positive and CD4 positive/
CD8 positive cells. The OX40 ligand binds CD134 protein on T cells and costimulates
proliferation. Crosslinking of CD134 with OX40 ligand on activated
B cells stimulates proliferation and antibody production suggesting a role in
B cell differentiation into plasma cells. Functionally, the CD134 molecule is
involved in T cell co-stimulation and T cell dependent antibody production.
IVD
IVD
Human tonsil: immunohistochemical staining for CD134 antigen using NCL-CD134. Note intense
membrane staining of activated T lymphocytes. Paraffin section.
Novocastra CD137
Clone S16
1 mL lyophilized NCL-CD137 P (HIER)
CD137 antigen, a member of the tumor necrosis factor receptor family, and
its ligand are reported to be expressed on activated T lymphocytes and on
antigen-presenting cells, respectively. This receptor/ligand system
regulates the activation, proliferation and survival of T and B lymphocytes
and monocytes through bidirectional signal transduction. Human CD137
antigen is reported to be expressed on activated B cells, Reed Sternberg
cells and peripheral blood monocytes but is absent from resting T cells. In
nonlymphoid cells, expression has been reported in blood vessel walls, on
the endothelial layer and on vascular smooth muscle cells. Soluble forms of
CD137 are reported at increased levels in sera of individuals with
rheumatoid arthritis. The expression of soluble CD137 lags behind that of
membrane bound CD137 by approximately 24 hours and it has been
proposed that as activation of lymphocytes through membrane-bound CD137
delivers a potent stimulatory signal then soluble CD137 may provide a
negative control mechanism for immune responses.
Human tonsil: immunohistochemical staining for CD137 antigen using NCL-CD137. Note intense
membrane staining of activated lymphoid cells. Paraffin section.
Novocastra CD138 (Syndecan 1)
Clone MI15
7 mL BOND ready-to-use PA0088 P (HIER)
Antigen Background
RUO*
The CD138 molecule is a transmembrane heparan sulphate glycoprotein
expressed at distinct stages of differentiation in normal lymphoid cells such
as pre-B cells, immature B cells and Ig-producing plasma cells as well as
being expressed in stratified and simple epithelia. The loss of CD138
expression from atypical cells is reported to be an early event during cervical
carcinogenesis whereas CD138 antigen expression shows a close
association with preserved epithelial morphology and differentiation,
however, the major utility of CD138 as a marker in immunohistochemistry is
the quantification of plasma cells.
IVD
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies