QF0159 Marketing Release Record

Novocastra Cytokeratin 20
Clone PW31 Reference Range
1 mL, 0.1 mL liquid NCL-L-CK20-561 P (HIER)
7 mL BOND ready-to-use PA0918 P (HIER)
Clone CK205
1 mL, 0.1 mL lyophilized NCL-CK20-543 P (HIER)
Clone Ks20.8 1 mL lyophilized NCL-CK20 P (HIER/Enzyme) W IVD
1 mL liquid NCL-L-CK20 P (HIER/Enzyme) W IVD
7 mL ready-to-use RTU-CK20 P (HIER/Enzyme) IVD
Clone PW31 was developed to produce superior staining on paraffin sections.
Antigen Background
Cytokeratin 20 has been demonstrated to be almost entirely confined to the
gastric and intestinal epithelium, urothelium and Merkel cells of the skin.
Cytokeratin 20 is less acidic than other type I cytokeratins and is of interest
due to its restricted tissue expression. In normal tissue, cytokeratin 20 is
expressed in intestinal epithelium, gastric foveolar epithelium, a number of
endocrine cells in the upper portions of the pyloric glands, urothelium and
Merkel cells in epidermis. In tumors it is reported, there is a marked
difference in the expression of cytokeratin 20 within different carcinomas.
Neoplasms expressing cytokeratin 20 are derived from normal epithelia
which themselves expressed cytokeratin 20. Colorectal carcinomas consistently
express cytokeratin 20, while gastric adenocarcinomas express
cytokeratin 20 to a lesser degree. Adenocarcinomas of the gall bladder and
bile duct, ductal cell adenocarcinomas of the pancreas, mucinous ovarian
tumors, Merkel cell tumors and transitional cell carcinomas have also been
reported to express cytokeratin 20.
Human colonic adenocarcinoma: immunohistochemical staining for cytokeratin 20 using
NCL-L-CK20-561. Note staining of malignant cells. Paraffin section.
Novocastra Cytokeratin (8/18)
Clone 5D3
1 mL, 0.1 mL lyophilized NCL-5D3 F P (Enzyme) C
1 mL liquid NCL-L-5D3 F P (Enzyme) C IVD
7 mL ready-to-use RTU-5D3 F P (Enzyme)
7 mL BOND ready-to-use PA0067 P (HIER)
Product Specific Information
In normal tissues, cytokeratins 8 and 18 are reported to be expressed in all
simple and glandular epithelium and in neoplastic tissues, they have been
reported to be expressed in adenocarcinomas and most squamous cell
carcinomas. These cytokeratins are absent from keratinizing squamous
carcinomas. Clone 5D3 reacts with human cytokeratin intermediate filament
proteins of 52.5 kD and 45 kD, identified as cytokeratins 8 and 18, respectively.
Clone 5D3 shares similar specificities to clone CAM5.2 (Angus B et al. Journal
of Pathology. 153: 377-384 (1987)).
IVD
IVD
IVD
IVD
IVD
IVD
Human breast: immunohistochemical staining for cytokeratins 8 and 18 using NCL-L-5D3.
Note intense cytoplasmic staining of ductal epithelial cells. Paraffin section.
Novocastra Cytokeratin (5/6/18)
Clone LP34
1 mL lyophilized NCL-LP34 F P (Enzyme) C
1 mL liquid NCL-L-LP34 F P (Enzyme) C IVD
7 mL ready-to-use RTU-LP34 F P (Enzyme)
Cytokeratins 5, 6 and 18 are reported to be expressed in a broad range of
human epithelial tissues, from simple glandular epithelia to stratified
squamous epithelia. These include epithelial cells that are ectodermal,
mesodermal, or endodermal in origin. These cytokeratins have been
reported to be expressed in tumor cells of epithelial origin and less
commonly of mesothelial origin. Non-epithelial tumors such as lymphomas
do not express these cytokeratins.
Product Specific Information
Clone LP34 reacts with human cytokeratin intermediate filament proteins 5, 6
and 18 on frozen tissue. The recognition of cytokeratin 18 on paraffin sections
using clone LP34 may be variable.
Novocastra Cytokeratin, Multi
Clone AE1, Clone AE3 cocktail
1 mL lyophilized NCL-AE1/AE3 F P (HIER)
1 mL liquid NCL-L-AE1/AE3 F P (HIER)
7 mL ready-to-use RTU-AE1/AE3 F P (HIER)
7 mL BOND ready-to-use PA0909 P (Enzyme)
See also Multi-Cytokeratin on page 84.
Novocastra Cytokeratin, Multi
(1/5/10/14)
Clone 34�E12
1 mL lyophilized NCL-CK34BE12 F P (HIER) W
7 mL ready-to-use RTU-CK34BE12 F P (HIER)
7 mL BOND ready-to-use PA0134 P (Enzyme)
See also Multi-Cytokeratin 1/5/10/14 on page 84.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
RUO*
RUO*
IVD
IVD
IVD
IVD
IVD
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Primary Antibodies