QF0159 Marketing Release Record

Human bone marrow: immunohistochemical staining for CD19 using NCL-L-CD19-163. Note
membrane staining of B cells. Paraffin section.
Novocastra CD20
Clone 7D1 Reference Range
1 mL lyophilized NCL-CD20-7D1 F P (HIER) W
Clone MJ1
1 mL, 0.1 mL lyophilized NCL-CD20-MJ1 F P (HIER)
7 mL BOND ready-to-use PA0906 P (HIER) IVD
Clone L26
1 mL lyophilized NCL-CD20-L26 F P (HIER) W IVD
1 mL liquid NCL-L-CD20-L26 F P (HIER) W IVD
7 mL ready-to-use RTU-CD20-L26 F P (HIER) IVD
Clone 7D1 was developed to produce superior manual staining on paraffin
sections.
Antigen Background
The CD20 antigen is a non-glycosylated phosphoprotein of approximately
33 kD which is expressed on normal and malignant human B cells and is
thought to act as a receptor during B cell activation and differentiation. CD20
antigen has been reported to be expressed on normal B cells from peripheral
blood, lymph node, spleen, tonsil, bone marrow, acute leukemias and chronic
lymphocytic leukemias.
Human centroblastic lymphoma: immunohistochemical staining for CD20 antigen using
NCL-CD20-7D1. Note intense membrane staining of B cells. Paraffin section.
IVD
RUO*
Novocastra CD21
Clone 2G9
1 mL, 0.1 mL lyophilized NCL-CD21-2G9 F P (HIER)
1 mL liquid NCL-L-CD21-2G9 F P (HIER) IVD
7 mL BOND ready-to-use PA0171 P (HIER) IVD
Antigen Background
CD21 antigen is a type I integral membrane glycoprotein of molecular weight
140 kD, which functions as the receptor for the C3d fragment of the third
complement component. The CD21 molecule, present on mature B cells, is
involved in transmitting growth-promoting signals to the interior of the B cell
and acts as a receptor for Epstein-Barr virus. CD21 antigen is reported to be
found in B cell chronic lymphocytic leukemias and in a subset of T cell acute
lymphocytic leukemias but is absent on T lymphocytes, monocytes and
granulocytes. CD21 antigen is also reported to be expressed in follicular
dendritic cells and in follicular and mantle cell lymphomas, mature leukemias
and lymphomas.
Human follicular lymphoma: immunohistochemical staining for CD21 antigen using NCL-CD21-
2G9. Note intense membrane staining of follicular dendritic cells. Paraffin section.
Novocastra CD22 (BL-CAM)
Clone FPC1
1 mL, 0.1 mL lyophilized NCL-CD22-2 P (HIER)
7 mL BOND ready-to-use PA0249 P (HIER)
Antigen Background
The CD22 antigen (BL-CAM) is a type 1 integral membrane glycoprotein with
a molecular weight of 130 to 140 kD. It is a heterodimer of two independently
expressed glycoprotein chains, present both on the membrane and in the
cytoplasm of B lymphocytes. Expression of the CD22 antigen is reported to
appear early in B cell lymphocyte differentiation at approximately the same
stage as that of the CD19 antigen expression. Surface antigen expression is
variable and may be lost upon differentiation. CD22 antigen is also reported to
be strongly expressed on hairy cell leukemias. It is absent on peripheral blood
T cells, T cell leukemias, granulocytes and monocytes.
IVD
RUO*
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
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Primary Antibodies