QF0159 Marketing Release Record

Ancillary Reagents
BOND Wash Solution 10X Concentrate
1 L AR9590 P
Components
IVD
BOND Wash Solution 10X Concentrate is a concentrated buffer solution
specifically for use on the automated BOND system. It is available in 1 L
quantities, and when diluted will make up 10 L of working solution.
Application
BOND Wash Solution is the only wash buffer that should be used in BOND
automated staining procedures. It is formulated for smooth and gentle
reagent flow under the BOND Covertile to help ensure that excess reagent
is removed from the tissue section before new reagent is added.
BOND Primary Antibody Diluent
500 mL AR9352 P
Components
BOND Primary Antibody Diluent is ready-to-use and available in a quantity
of 500 mL.
Application
BOND Primary Antibody Diluent is specifically for diluting concentrated
primary antibodies for use on the automated BOND system. It is not intended
for the reconstitution of lyophilized reagents.
BOND DAB Enhancer
30 mL AR9432 P
Components
IVD
IVD
BOND DAB Enhancer is a heavy metal solution for use on the automated BOND
system. The no-mix, ready-to-use format simplifies laboratory workflow.
Application
BOND DAB Enhancer changes the color of the DAB reaction deposit from
golden to dark brown, providing an increase in contrast between
chromogen-specific staining and the slide back drop. This can assist in
qualitative identification of antigens.
Staining without DAB Enhancer. Human tonsil stained for CD20 with NCL-CD20-MJ1 using
BOND Polymer Refine Detection.
Staining with DAB Enhancer. Human tonsil stained for CD20 with NCL-CD20-MJ1 using BOND
Polymer Refine Detection.
BOND Dewax Solution
1 L AR9222 P
Components
BOND Dewax Solution is a deparaffinization solution specifically designed
for use on the automated BOND system. It is provided ready-to-use in 1 L
bottles and can be poured directly into the appropriate bulk reagent
container on the instrument.
Application
The use of BOND Dewax Solution allows paraffin wax to be removed from
tissue sections before rehydration and staining on BOND. It is specially
formulated to be compatible with the automated BOND system, and
efficiently removes wax from slides while retaining the integrity of tissue
antigens and probe binding sites. BOND Dewax Solution is much less
harmful than alternative deparaffinization solutions such as xylene.
BOND Enzyme Pretreatment Kit
1 kit AR9551 P
Components
IVD
IVD
BOND Enzyme Concentrate, 1 mL
BOND Enzyme Diluent, 200 mL
3 x BOND Open Containers, 7 mL
The enzyme is diluted before use in the BOND Open Containers supplied.
The diluted enzyme solution is used for enzymatic digestion on the
automated BOND system.
Application
Immunohistochemistry (IHC)
The BOND Enzyme Pretreament Kit can be used for enzymatic digestion on
formalin-fixed, paraffin-embedded tissue sections to assist in epitope
exposure. Enzymatic pretreatment improves the staining of some antibodies
by exposing epitopes within tissue that have been masked during fixation.
Application
In Situ Hybridization (ISH)
The diluted enzyme solution can also be used for ISH. Enzymatic digestion of
tissue assists in the penetration of probes and facilitates binding.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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BOND