QF0159 Marketing Release Record

Primary Antibodies
Novocastra B Cell Specific Octamer
Binding Protein-1 (BOB-1)
Clone TG14
1 mL liquid NCL-L-BOB-1 ASR
7 mL BOND ready-to-use PA0558 P (HIER) IVD
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra bcl-2 Oncoprotein
Clone 3.1 Reference Range
1 mL lyophilized NCL-bcl-2-486 P (HIER) W
Clone bcl-2/100/D5
1 mL lyophilized NCL-bcl-2 F P (HIER) W IVD
1 mL liquid NCL-L-bcl-2 F P (HIER) W IVD
7 mL ready-to-use RTU-bcl-2 F P (HIER) IVD
7 mL BOND ready-to-use PA0117 P (HIER) IVD
Clone 3.1 was developed to produce superior staining on paraffin sections.
Antigen Background
Bcl-2 is a member of a family of proteins that are involved in apoptosis. Bcl-2
is an integral inner mitochondrial membrane protein of 25 kD and has a wide
tissue distribution. It is considered to act as an inhibitor of apoptosis. For this
reason, bcl-2 expression is inhibited in germinal centers where apoptosis
forms part of the B cell production pathway. In 90 percent of follicular
lymphomas a translocation occurs which juxtaposes the bcl-2 gene at 18q21,
to an immunoglobulin gene. This t(14;18) translocation can deregulate gene
expression and bcl-2 over-expression can be demonstrated immunohistochemically
in the vast majority of follicular lymphomas.
Human breast carcinoma: immunohistochemical staining for bcl-2 oncoprotein using
NCL-bcl-2-486. Note membrane staining of tumor cells and also cytoplasmic staining
in a small proportion of these. Paraffin section.
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IVD
Novocastra Bcl-3 Oncoprotein
Clone 1E8
1 mL lyophilized NCL-Bcl-3 F P (HIER)
RUO*
Bcl-3 was first identified as a putative proto-oncogene and was originally
isolated through its involvement in the translocation event t(14;19) where it is
highly expressed in a subset of chronic lymphocytic leukemias and other
B cell neoplasms. The Bcl-3 gene product is also thought to play a role in the
immune system through its interactions with the NF-kappaB family of
transcription factors to enhance proliferation and to act as a transcription
cofactor. More specifically, Bcl-3 oncoprotein appears to regulate the
activity of homodimeric NF-kappaB p50 subunit and a closely-related
homolog, p52, in a phosphorylation-dependent manner. Although to date, no
immunohistochemistry data has been published, Bcl-3 mRNA is found in a
number of tissues, including spleen and other lymphoid tissues.
Human tonsil: immunohistochemical staining for Bcl-3 oncoprotein using NCL-Bcl-3. Note
nuclear staining of a proportion of follicular cells, parafollicular cells and mucosa. Paraffin
section.
Novocastra Bcl-6 Oncoprotein
Clone LN22 Reference Range
1 mL, 0.1 mL liquid NCL-L-Bcl-6-564 P (HIER)
7 mL BOND ready-to-use PA0204 P (HIER)
Clone P1F6
1 mL, 0.1 mL lyophilized NCL-Bcl-6 F P (HIER) RUO*
Clone LN22 was developed to produce superior staining compared to clone
P1F6 on paraffin sections.
Antigen Background
Bcl-6 is a proto-oncogene that encodes a Kruppel-type zinc-finger protein of
95 kD and shares homology with other transcription factors. Bcl-6 protein is
mainly expressed in normal germinal center B cells and related lymphomas.
It has been shown that the Bcl-6 proto-oncogene is involved in chromosome
rearrangements at 3q27 in non-Hodgkin's lymphomas and Bcl-6 rearrangements
have also been detected in 33 to 45 percent of diffuse large B cell
lymphomas. Immunohistochemistry has been reported to show the Bcl-6
gene product to be detectable in follicular lymphomas, diffuse large B cell
lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant
Hodgkin's disease.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD
IVD