QF0159 Marketing Release Record

BOND
Detection Systems
BOND Polymer Refine Detection
300 Tests (100 μL dispense) or
200 Tests (150 μL dispense) DS9800 P
Components
A state-of-the-art Compact Polymer detection system for use in both
immunohistochemistry and chromogenic in situ hybridization. Small
multifunctional linkers enhance tissue penetration, producing unsurpassed
sensitivity. The system is biotin-free.
BOND Polymer Refine Detection contains a peroxide block, post primary,
polymer reagent, DAB chromogen and hematoxylin counterstain. It is
supplied ready-to-use for the automated BOND system.
Application
Immunohistochemistry (IHC)
Primary antibody binding to tissue sections can be visualized using BOND
Polymer Refine Detection, where it provides intense, high resolution
staining. A range of BOND ready-to-use primary antibodies are available, or
alternatively, use antibody concentrates diluted with BOND Primary
Antibody Diluent (AR9352).
Colon mucosa: immunohistochemical staining with BOND ready-to-use Cytokeratin 8/18 (5D3)
(PA0067) using BOND Polymer Refine Detection.
Application
Chromogenic in Situ Hybridization (ISH)
BOND Polymer Refine Detection produces highly specific, sensitive and
reproducible demonstration of nucleic acid sequences through controlled
hybridization reactions.
BOND Polymer Refine Red Detection
100 Tests DS9390 P
Leica CV Ultra Mounting Media (Product Code 14070936261) is recommended
for use with the BOND Polymer Refine Red Detection System.
BOND Polymer Refine Red Detection is an IVD labeled red detection system
for the automated BOND system. BOND Polymer Refine Red Detection is
biotin-free, utilizing alkaline phosphatase (AP)-linked compact polymers to
provide enhanced tissue penetration and unsurpassed reagent sensitivity. It
contains post primary, polymer reagent, Fast Red chromogen, and
hematoxylin counterstain and is supplied in a convenient, ready-to-use
format.
BOND Polymer Refine Red is compatible with version 4.0 software and
version 26.0 database update or greater.
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IVD
For detailed information on all products please visit our website:
www.leica-microsystems.com
IVD
Human skin stained for melanoma marker HMB45 with NCL-HMB45 using BOND Polymer
Refine Red Detection. Note intense cytoplasmic staining of melanocytes in contrast to the
brown endogenous melanin.
BOND Intense R Detection
300 Tests (100 μL dispense) or
200 Tests (150 μL dispense) DS9263 PF
Components
BOND Intense R Detection is a peroxidase detection system optimized for
use on the automated BOND system and is ideal for research applications. It
contains a peroxide block, streptavidin/peroxidase conjugate, DAB
chromogen and hematoxylin counterstain. Users must supply a biotinylated
secondary antibody of their choice.
Application
By allowing a free choice of biotinylated secondary antibody, BOND Intense
R Detection is ideal for the detection of primary antibodies from any species.
Research applications such as IHC staining of mouse tissues can be
accommodated in this manner. The intense deposition of DAB reaction product
produces strong immunostaining.
BOND Research Detection
300 Tests (100 μL dispense) or
200 Tests (150 μL dispense) DS9455 RUO*
Components
The BOND research functionality is created together with a research dongle
and an "open" BOND Research Detection from Leica Microsystems. This
open detection system consists of six standard 30 mL open containers in a
reagent tray. The tray is registered on BOND like any other detection system
(one barcode only), but each of the containers can be configured with
reagent of the user's choice.
Application
BOND Research Detection offers researchers the ability to tailor applications
and fully automate for ease of use.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
RUO*