QF0159 Marketing Release Record

Novocastra Immunoglobulin M
Clone 8H6 Reference Range
1 mL, 0.1 mL liquid immunoglobulin M NCL-L-IgM P (HIER) W
IVD
Clone 8H6 was developed to produce reduced background staining that is
associated with polyclonal antibodies on paraffin sections.
Antigen Background
IgM, together with IgD, is the major immunoglobulin expressed on the surface
of B cells and normally constitutes about 10 per cent of serum
immunoglobulin. IgM antibody is prominent in early immune responses to
most antigens and predominates in certain antibody responses such as
‘natural’ blood group antibodies.
Human tonsil: immunohistochemical staining for immunoglobulin M using NCL-L-IgM.
Note staining of follicular dendritic cells, mantle zone B cells and intense staining of plasma
cells. Paraffin section.
Novocastra Immunoglobulin Antibodies
Polyclonal
1 mL lyophilized immunoglobulin A
NCL-IgAp P (Enzyme) W IVD
The basic structure of immunoglobulin (Ig) molecules is a tetramer of two
light chains and two heavy chains linked by disulfide bonds. There are two
types of light chains, kappa and lambda, each composed of a constant
domain (CL) and a variable domain (VL). There are five types of heavy
chains: alpha, delta, epsilon, gamma and mu, all consisting of a variable
domain (VH) and three (in alpha, delta and gamma) or four (in epsilon and
mu) constant domains (CH1 to CH4).
Product Specific Information
NCL-IgAp, NCL-IgDp, NCL-IgGp and NCL-IgMp have each been solid-phase
absorbed to remove cross-reactivity.
Novocastra Inhibin (Alpha)
Clone AMY82
1 mL, 0.1 mL liquid NCL-L-InhibinA P (HIER)
7 mL BOND ready-to-use PA0110 P (HIER)
Antigen Background
Inhibins and activins are members of the transforming growth factor beta
(TGF ) family of cytokines. Inhibins are heterodimers consisting of a common
-subunit linked to either a A subunit ( - A, forming inhibin A) or a B subunit
( - B, forming inhibin B). Activins share the -subunit with the inhibins and
may be homo or heterodimers of -subunits forming activin A ( A- A), activin
AB ( A- B) or activin B ( B- B). The expression of the -subunit, and
therefore of inhibins appears to be more restricted than that of the -subunit,
and therefore of activins. Inhibins and activins play a role in the regulation of
pituitary follicle stimulating hormone (FSH) secretion. The actions of inhibins
and activins are thought to oppose one another, with inhibins suppressing
FSH secretion and activins stimulating FSH secretion. Inhibins are secreted
by granulosa cells in female follicles and Sertoli cells of the testis in the male.
Inhibins are thought to have local regulatory roles in a variety of tissues, in
addition to the ovary, including the brain, adrenal glands, bone marrow, fetus
and placenta.
Human testis: immunohistochemical staining for Inhibin Alpha using NCL-L-InhibinA. Paraffin
section.
Novocastra Insulin
Clone 2D11-H5
1 mL, 0.1 mL lyophilized NCL-INSULIN P
7 mL BOND ready-to-use PA0620 P IVD
Insulin is a hormone secreted by the beta cells of the islets of Langerhans in
the pancreas. It promotes glycogen storage, formation of triglycerides, and
synthesis of protein and nucleic acids. Reports of immunocytochemical
investigation reveal the presence of insulin in the cytoplasm of certain islet
tumors. However, in some instances insulin-positive granules are sparse
and form a margin against the cell membrane.
Human pancreas: immunohistochemical staining for insulin-containing cells using NCL-
INSULIN. Note intense cytoplasmic staining of the beta cells of the islets of Langerhans and of
the tumor cells (center). Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
IVD
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Primary Antibodies