QF0159 Marketing Release Record

Novocastra Nitric Oxide Synthase
Antibodies
Clone NOS-125
1 mL lyophilized Nitric Oxide Synthase-1
NCL-NOS-1 P (HIER) IVD
Clone RN5
1 mL lyophilized Nitric Oxide Synthase-3
NCL-NOS-3 F P (HIER) RUO*
Human nitric oxide synthases are a family of enzymes responsible for the
synthesis of nitric oxide from L-arginine and molecular oxygen. There are at
least three nitric oxide synthases; NOS-1, also known as neuronal NOS or
nNOS, NOS-2, which is referred to as inducible NOS or iNOS and NOS-3,
also known as endothelial NOS or eNOS. As suggested by their nomenclature,
these enzymes have different cellular distribution and are subjected
to different regulatory mechanisms. NOS-3 is reported to be constitutively
expressed and produces picomolar quantities of nitric oxide (NO) which play
a role in signal transmission resulting in physiological effects. In the
gastrointestinal tract, NO is reported to play a protective role where it has
direct microbiocidal properties and acts as a first line of mucosal defence in
the stomach. The function of NO in tumor development, promotion and
progression is unclear. The effects may be both beneficial but also
detrimental to those individuals with gastric cancer where it is reported that
NO supports tumor progression through the creation of neovasculature.
Human small intestine: immunohistochemical staining for nitric oxide synthase-1 using
NCL-NOS-1. Note cytoplasmic staining of enteric ganglia. Paraffin section.
Novocastra nm23 Protein
Clone 37.6
1 mL lyophilized NCL-nm23 FPW
The nm23 gene was first identified by differential screening of mousederived
melanoma cell lines of high and low metastatic potential. Two
human homologs of the nm23 gene have been isolated and designated
nm23-H1 and nm23-H2. The products of these genes have been identified as
nucleoside diphosphate kinase A (NDPK-A) and nucleoside diphosphate
kinase B (NDPK-B), respectively. nm23-H1 and nm23-H2 are metastasissuppressor
genes implicated in the control of the metastatic process of
malignant cells.
Product Specific Information
NCL-nm23 reacts with H1 strongly and only weakly with the H2 homolog.
IVD
Novocastra NS3 (Hepatitis C virus)
Clone MMM33
1 mL, 0.1 mL lyophilized NCL-HCV-NS3 F P (HIER)
See also Hepatitis C virus (NS3) on page 70.
Novocastra Oct-2
Clone Oct-207 Reference Range
1 mL, 0.1 mL lyophilized NCL-OCT2 F P (HIER)
7 mL BOND ready-to-use PA0532 P (HIER)
Antigen Background
Oct-2 is a transcription factor belonging to the POU homeo-domain family that
binds to the Ig gene octamer sites regulating B cell specific genes. It is
dependent on the activity of B cell restricted coactivators such as BOB.1/
OBF.1. Oct-2 protein expression is not restricted to B cells, although
expression levels are much higher in these cells. Reports indicate that
germinal center B cells shows higher expression for Oct-2 and BOB.1/OBF.1.
In addition, Oct-2 expression is reported to be significantly greater in
germinal center derived lymphomas, although other B cell lymphomas also
display high levels of expression. Reed Sternberg (RS) cells represent the
malignant cells in classical Hodgkin's disease and are derived from germinal
center B cells. In a number of these cases, cells do not express immunoglobulin
due to the presence of crippling mutations within the Ig genes. As Ig
gene expression in B cells also requires an interaction between octamer
sites and the transactivating factors Oct-2 and BOB.1, the absence of both
Oct-2 and BOB.1 expression represents a novel mechanism for
immunoglobulin gene deregulation in RS cells.
Human tonsil: immunohistochemical staining for Oct-2 gene product using NCL-OCT2.
Note intense nuclear staining of mainly germinal center B cells. Paraffin section.
Novocastra Oct-3/4
Clone N1NK
1 mL, 0.1 mL liquid NCL-L-Oct3/4 P (HIER) W
7 mL BOND ready-to-use PA0934 P (HIER)
Antigen Background
Oct3/4 is a member of the POU homeodomain family of transcription factors,
which is expressed by embryonic stem cells and germ cells. A critical amount
of Oct3/4 levels are associated with loss of pluripotency. Oct3/4 has been
proposed as a useful marker for germ cell tumors which exhibit features of
pluripotentiality, including seminoma/dysgerminoma and embryonal
carcinoma, and establishing a germ cell origin for some metastatic tumors of
uncertain primary tumor.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
IVD
IVD
RUO*
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Primary Antibodies