QF0159 Marketing Release Record

Human squamous cell carcinoma, floor of the mouth: immunohistochemical staining for CD44
variant 3 using NCL-CD44v3. Note intense membrane staining of tumor cells. Paraffin section.
Novocastra CD45
Clone X16/99 Reference Range
1 mL, 0.1 mL lyophilized NCL-LCA F P (HIER) C
7 mL BOND ready-to-use PA0042 P (HIER)
RP2/18, Clone RP2/22
IVD
1 mL lyophilized NCL-LCA-RP FP RUO*
1 mL liquid NCL-L-LCA-RP FP IVD
7 mL ready-to-use RTU-LCA-RP FP RUO*
Antigen Background
The CD45 antigen (leukocyte common antigen) is a family of five or more high
molecular weight glycoproteins present on the surface of the majority of the
human leukocytes (including lymphocytes, monocytes and eosinophils) but
absent from erythrocytes and platelets. Various isoforms of CD45 are
generated by alternative splicing of three exons. Expression of CD45 is
necessary for signalling through the T cell receptor.
Product Specific Information
NCL-LCA-RP is a cocktail of two antibodies, clone RP2/18 and RP2/22. The
heat induced epitope retrieval (HIER) technique may enhance staining in
some cases with NCL-LCA-RP, NCL-L-LCA-RP and RTU-LCA-RP.
Human stomach, B cell lymphoma: immunohistochemical staining for CD45 antigen using
NCL-L-LCA-RP. Note intense membrane staining of tumor cells. Paraffin section.
IVD
Novocastra CD45RO
Clone UCHL1
1 mL, 0.1 mL lyophilized NCL-UCHL1 F P (HIER) C
1 mL liquid NCL-L-UCHL1 F P (HIER) C IVD
7 mL ready-to-use RTU-UCHL1 F P (HIER) RUO*
7 mL BOND ready-to-use PA0146 P (HIER) IVD
Antigen Background
The CD45RO molecule, a 180 kD isoform of CD45, is reported to be expressed
on 48 percent of peripheral blood T lymphocytes, 37 percent of CD4 positive
lymphocytes, 80 percent of thymocytes and on the majority of T cell
malignancies. Monocytes and granulocytes show surface expression of the
antigen whereas tissue macrophages exhibit cytoplasmic expression.
Product Specific Information
The heat induced epitope retrieval (HIER) technique may enhance staining in
some cases.
Novocastra CD56 (NCAM)
Clone 1B6 Reference Range
1 mL, 0.1 mL lyophilized NCL-CD56-1B6 P (HIER) W
1 mL liquid NCL-L-CD56-1B6 P (HIER) W IVD
7 mL ready-to-use RTU-CD56-1B6 P (HIER) IVD
Clone CD564
1 mL lyophilized NCL-CD56-504 ASR
7 mL BOND ready-to-use PA0191 P (HIER) IVD
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra CD57
Clone NK-1
1 mL, 0.1 mL lyophilized NCL-NK1 FP IVD
7 mL ready-to-use RTU-NK1 FP IVD
7 mL BOND ready-to-use PA0443 P (HIER)
Antigen Background
The CD57 glycoprotein, also known as HNK-1, has a molecular weight of 110
kD. It is found on a subset of mononuclear cells with natural killer activity and
on neuroectodermal cells expressing myelin-associated glycoprotein. Many
cells which co-express CD57 and CD8 proteins are a subset of suppressor/
cytotoxic T cells. These cells play a role in the rejection of grafts in acute graft
versus host disease. The CD57 molecule is not expressed on erythrocytes or
platelets.
Product Specific Information
IVD
Enzyme pretreatment may enhance staining in some cases.
RUO*
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
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Primary Antibodies