QF0159 Marketing Release Record

Novocastra Alpha-1-Antitrypsin
Polyclonal
1 mL lyophilized NCL-A1Ap ASR
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra Alpha-Actinin
Clone RBC2/1B6
1 mL lyophilized NCL-alpha-ACT FW
Alpha-actinin is a rod-like cytoskeletal protein belonging to the same family
as spectrin, dystrophin and utrophin. In skeletal muscle, alpha-actinin is
located in the Z band/disc and cross-links with F-actin in this region. Muscle
tissues show the presence of abundant threadlike particles, known as
nemaline bodies, in the myofibers. Electron microscopy studies have shown
that the nemaline rods have a lattice structure similar to that of the Z discs
and the rods are thought to be lateral polymers of the Z discs.
Novocastra Alpha-Catenin
RUO*
Clone 25B1
1 mL lyophilized NCL-A-CAT F P (HIER) W
RUO*
Alpha-catenin, which shows some homology with vinculin, appears to play a
role in tumor invasion and metastasis through the dysfunction of E-cadherin.
Normal epithelium of the esophagus, stomach and colon express alphacatenin
strongly, without exception. However, in primary tumors of these
tissues its expression is frequently reduced. It has been suggested that
some human cancer cells may have impaired E-cadherin-mediated cell
adhesiveness as a result of the downregulation of alpha-catenin expression.
Abnormalities in the expression of alpha-catenin seem to associate with
malignant cellular features and disease progression in prostate cancer. In
one study of several tumor-related proteins in breast cancers, it was shown
that alpha and beta-catenins could be re-expressed in metastatic tissue. Reexpression
of these adhesion molecules by tumor cells after release from
the primary site may be important and perhaps necessary for cells to adhere
in remote organs.
Human appendix: immunohistochemical staining for alpha catenin using NCL-A-CAT.
Note intense membrane staining of the mucosal epithelial cells. Paraffin section.
Novocastra Alpha Fetoprotein
Clone C3
1 mL, 0.1mL lyophilized NCL-AFP FP
7 mL BOND ready-to-use PA0963 P
Antigen Background
Alpha fetoprotein (AFP) is an oncofetal antigen of 70 kD found in body fluids
which if detected in high concentrations has clinical implications. AFP is
expressed in fetal liver but is not present under normal circumstances in
healthy adult tissues. It is reported to be expressed in a proportion of germ
cell tumors, with high frequency in yolk sac tumors.
Human fetal liver: immunohistochemical staining for alpha fetoprotein using NCL-AFP.
Note cytoplasmic staining of hepatocytes. Paraffin section.
Novocastra Alpha Smooth Muscle Actin
(SMA)
Clone �sm-1
1 mL lyophilized NCL-SMA F P (Enzyme) W
7 mL ready-to-use RTU-SMA F P (Enzyme)
7 mL BOND ready-to-use PA0943 P IVD
Antigen Background
Cytoplasmic actins are part of the microfilament system of cytoskeletal
proteins. Smooth muscle actin is found in vascular walls, intestinal
muscularis mucosae and muscularis propria and in the stroma of various
tissues. It is also reported to be expressed in myofibroblasts and myoepithelial
cells and antibodies to SMA are reported to be a useful tool for the
identification of leiomyomas, leiomyosarcomas and pleomorphic adenomas.
When such antibodies are used in a differential diagnostic situation they must
be used within a panel of antibodies together with the clinical presentation
data and interpreted by a pathologist.
Product Specific Information
Enzyme pretreatment may enhance staining in some cases.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
IVD
IVD
/21
Primary Antibodies