QF0159 Marketing Release Record

Primary Antibodies
Human testis: immunohistochemical staining for glutathione S-transferase mu using
NCL-GSTmu-437. Note cytoplasmic staining of sertoli cells. Paraffin section.
Novocastra Gonadotropin-Releasing
Hormone Receptor
Clone A9E4
1 mL lyophilized NCL-GnRHR P (HIER)
Gonadotropin-releasing decapeptide hormone is the key mediator in the
integration of the neural and endocrine systems. It regulates the reproductive
cycle in both sexes. Gonadotropin-releasing hormone (GnRH) stimulates
the gonadotrophs of the anterior pituitary to secrete luteinizing hormone as
well as follicle stimulating hormone via specific receptors. The presence of
high-affinity binding sites for GnRH has been reported in luteal and
granulosa cells as well as in ovarian cell membrane preparations. These
receptors have similar binding characteristics to those of GnRH receptors in
the anterior pituitary.
Product Specific Information
NCL-GnRHR is specific for the extracellular domain of the GnRH receptor.
Novocastra GPIb (CD42b)
Clone MM2/174
1 mL lyophilized NCL-CD42b F P (HIER)
See also CD42b (GPIb) on page 40.
Novocastra GPIIb/IIIa (CD41)
Clone M148
1 mL lyophilized NCL-CD41 F
See also CD41 (GPIIb/IIIa) on page 40.
Novocastra GPIIIa (CD61)
Clone 2f2
1 mL lyophilized NCL-CD61-308 ASR
7 mL BOND ready-to-use PA0308 P (HIER)
See also CD61 (GPIIIa) on page 42.
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RUO*
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Novocastra Granulysin
Clone RJT48
1 mL liquid NCL-L-Granulysin P (HIER)
Granulysin is a member of the Saposin-like protein (SAPLIP) family. It is a
lytic protein made up of two fragments, 15 kD and 9 kD, reported to be
expressed selectively by NK cells and activated T cells. Granulysin protein is
highly homologous to a porcine antimicrobial and antitumor protein called
NK lysine and to amebapores, polypeptides used by amoebae to kill
bacterial prey. Granulysin disrupts artificial liposomes and cell membranes,
damages mitochondria and activates caspase 9 to induce apoptosis in
nucleated cells.
Human NK cell lymphoma: immunohistochemical staining for granulysin using NCL-L-
Granulysin. Note intense granular cytoplasmic staining on a proportion of NK cells. Paraffin
section.
Novocastra Granzyme B
Clone 11F1
1 mL, 0.1 mL lyophilized NCL-GRAN-B P (HIER)
1 mL liquid NCL-L-GRAN-B P (HIER)
7 mL ready-to-use RTU-GRAN-B P (HIER)
7 mL BOND ready-to-use PA0291 P (HIER)
Antigen Background
Granzymes are neutral serine proteases which are stored in specialized lytic
granules of cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells.
These CTL and NK cells are heavily involved in the elimination of neoplastic
and virally infected cells. Secretory granules containing perforin and
granzymes are instrumental in undertaking cytolytic activity. Granzyme B is
understood to enter a target cell through a perforin pore-formed channel to
induce DNA fragmentation and apoptosis. Granzyme B has also been
described in neoplastic CTL and NK cells.
Hodgkin's disease: immunohistochemical staining for granzyme B using NCL-L-GRAN-B.
Note granular cytoplasmic staining in a number of Reed Sternberg cells. Paraffin section.
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Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
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