QF0159 Marketing Release Record

Novocastra Tyrosinase
Clone T311
1 mL lyophilized NCL-TYROS F P (HIER)
1 mL liquid NCL-L-TYROS F P (HIER)
7 mL ready-to-use RTU-TYROS F P (HIER)
7 mL BOND ready-to-use PA0322 P (HIER)
Antigen Background
The biosynthesis of melanin within melanocytes involves a family of enzymes,
a key member of which is tyrosinase. Tyrosinase deficiency is associated
with various forms of albinism and in particular oculocutaneous albinism. Ltyrosine
is the initial substrate for melanin biosynthesis and its conversion to
dopaquinone is catalyzed by tyrosinase whose expression is reported in
melanocytes and melanomas.
Human malignant melanoma: immunohistochemical staining for tyrosinase using RTU-TYROS.
Note cytoplasmic staining of tumor cells. Paraffin section.
Novocastra Tyrosinase-Related
Protein-1
Clone G3E6
1 mL lyophilized NCL-TRP-1 P (HIER)
Tyrosinase-related protein-1 (TRP-1) is a member of a family of proteins
which are involved in melanin biosynthesis. The catalytic function of TRP-1
has not been fully resolved but the enzyme appears to be important in the
oxidation of 5,6-dihydroxyindole-2-carboxylic acid to form a high molecular
weight pigmented biopolymer. In mammals, there are two basic types of
melanin, the brown-black eumelanin and the reddish-yellow phaeomelanin.
The concentrations of each are variable and are not related to skin type. In
skin exposed to suberythemal doses of UVB, an increase in the number of
melanocytes expressing TRP-1 and TRP-2 is reported with no increase in the
number of tyrosinase-expressing melanocytes. In normal, untreated skin the
number of melanocytes that express either TRP-1, TRP-2 or tyrosinase are
similar irrespective of skin type. TRP-1 is also reported to be expressed in
more than 50 percent of choroidal melanocytes in the adult eye.
Human malignant melanoma: immunohistochemical staining for tyrosinase-related protein-1
using NCL-TRP-1. Note cytoplasmic staining of tumor cells. Paraffin section.
IVD
RUO*
IVD
IVD
IVD
Novocastra Tyrosine Hydroxylase
Clone 1B5
1 mL lyophilized NCL-TH P (HIER) W
Tyrosine hydroxylase is the first enzyme in catecholamine (CA) biosynthesis
and catalyzes the conversion of L-tyrosine to L-DOPA. Tyrosine hydroxylase
is reported to be expressed in all CA neurons. Despite the abundant data
about the distribution of catecholaminergic neurons in a wide variety of
species, data on their distribution in the human brain is less comprehensive.
However, one such study has reported that tyrosine hydroxylase products in
the substantia nigra were restricted to neural bodies, axons and dendrites.
These in turn were restricted to the third decade of life and their number
increased in this location with age. This finding may be related to ageing of
melanin-pigmented neurons.
Product Specific Information
NCL-TH is reactive with tyrosine hydroxylase in human, mouse and rat brain
tissue.
Human midbrain: immunohistochemical staining of tyrosine hydroxylase enzyme using
NCL-TH. Note cytoplasmic staining of catecholaminergic cells and their processes.
Paraffin section (Peroxidase substrate: nickel DAB, Counterstain: eosin).
Novocastra Utrophin
Clone DRP3/20C5
2.5 mL, 1 mL lyophilized NCL-DRP2 FE
Utrophin, located on chromosome 6, is a ubiquitously expressed homologue
of dystrophin and is known as dystrophin-related protein. In normal muscle,
utrophin is restricted to neuromuscular junctions. However, in dystrohindeficient
muscle, utrophin expression is reported to be upregulated and
appears around the periphery of most fibres. Utrophin has a role as a cell
anchoring molecule. The amino terminal region of utrophin binds to the actin
cytoskeleton, acting as an intracellular anchor whereas the carboxyl
terminal regions bind to a group of proteins anchored in the cell membrane.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
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Primary Antibodies