QF0159 Marketing Release Record

Novocastra Cytokeratin 7
Clone RN7 Reference Range
1 mL, 0.1 mL liquid NCL-L-CK7-560 P (HIER)
7 mL BOND ready-to-use PA0942 P (HIER)
Clone OV-TL 12/30
1 mL lyophilized NCL-CK7-OVTL F P (HIER/Enzyme) W IVD
1 mL liquid NCL-L-CK7-OVTL F P (HIER/Enzyme) W IVD
7 mL ready-to-use RTU-CK7-OVTL F P (HIER/Enzyme) IVD
Clone RN7 was developed to produce superior staining on paraffin sections.
Antigen Background
Cytokeratins are intermediate filament proteins present in epithelial cells.
They are expressed in a tissue-specific manner in normal organs and the
tumors that arise from them. Cytokeratin 7 belongs to the neutral basic type B
subfamily of cytokeratins. Its distribution is confined to glandular and
transitional epithelia. Cytokeratin 7 is reported to be expressed in abun-dance
in cultured bronchial and mesothelial cells but only at lower levels in cultured
epidermal cells. The predicted amino acid sequence of this keratin has
revealed a striking difference between this keratin and the type II keratins
expressed in epidermal cells. Cytokeratin 7 has been reported in
adenocarcinomas of the lung, breast, endometrium, ovary, thyroid as well as
in carcinomas of the bladder and chromophobe renal cell carcinoma.
Cytokeratin 7 and Cytokeratin 20 expression have been reported to show
characteristic patterns on primary and metastatic lung and colorectal
adenocarcinomas.
Product Specific Information
Where clone OV-TL 12/30 can produce unwanted staining of endothelial cells,
clone RN7 does not stain these cell types. The choice of epitope retrieval,
heat or enzyme, to provide the best result with clone OV-TL 12/30 should be
determined by the user. Clones RN7 and OV-TL 12/30 react with the human
cytokeratin intermediate filament protein (54 kD) identified as cytokeratin 7.
Infiltrating lobular carcinoma of breast: immunohistochemical staining for cytokeratin 7
antigen using NCL-L-CK7-560. Note intense membrane and cytoplasmic staining of malignant
cells. Paraffin section.
Novocastra Cytokeratin 8
Clone TS1
1 mL, 0.1 mL lyophilized NCL-CK8-TS1 F P (HIER)
1 mL liquid NCL-L-CK8-TS1
ASR
7 mL ready-to-use RTU-CK8-TS1 F P (HIER) IVD
7 mL BOND ready-to-use PA0567 P (HIER) IVD
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
IVD
IVD
RUO*
Novocastra Cytokeratin 10
Clone LHP1
1 mL lyophilized NCL-CK10 F P (Enzyme)
Cytokeratin 10 is found in suprabasal layers of keratinizing stratified
epithelia. It is also found in a variable number of cells in suprabasal layers of
non-keratinizing stratified epithelia and is reported to be expressed in more
differentiated areas of some squamous carcinomas. Cytokeratin 10 is found
in various normal epithelia, including the anal canal, foot sole epidermis and
epidermises of other locations. Cytokeratin 10 is reported to be expressed in
ductal carcinoma of breast and squamous cell carcinoma of the ano-rectal
region.
Product Specific Information
NCL-CK10 reacts with the human cytokeratin intermediate filament protein
(56.5 kD) identified as cytokeratin 10.
Novocastra Cytokeratin 13
Clone KS-1A3
0.5 mL lyophilized NCL-CK13 F P (HIER) W
Cytokeratin 13 is expressed as a major component of squamous, nonkeratinised
epithelium, transitional epithelium, pseudostratified epithelium
and myoepithelium. It is reported to be expressed in carcinomas of the
trachea, apocrine and eccrine sweat glands, salivary glands, reserve cells
of endocervical glands, bladder, ectocervix, tongue, esophagus, anal canal
and the basal layer of keratinised epidermis.
Product Specific Information
NCL-CK13 reacts with the acidic intermediate filament protein (54 kD)
identified as cytokeratin 13.
Novocastra Cytokeratin 14
Clone LL002 Reference Range
1 mL, 0.1 mL lyophilized NCL-LL002 F P (HIER)
1 mL liquid NCL-L-LL002 F P (HIER) IVD
7 mL ready-to-use RTU-LL002 F P (HIER) IVD
Antigen Background
RUO*
RUO*
Cytokeratins 14 and 5 are useful to distinguish stratified epithelial cell types
from simple epithelial cell types. Cytokeratin 14 has been reported to be
expressed in neoplasms of squamous cell origin.
Product Specific Information
Clone LL002 reacts with the human cytokeratin intermediate filament protein
(50 kD) identified as cytokeratin 14.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
/53
Primary Antibodies