QF0159 Marketing Release Record

Novocastra Factor XIIIa (Blood
Coagulation Factor XIIIa)
Clone E980.1
1 mL lyophilized NCL-FXIIIa P (HIER) IVD
7 mL BOND ready-to-use PA0449 P (HIER)
Antigen Background
Factor XIIIa also known as fibrinoligase and fibrin-stabilizing factor, is the last
enzyme generated in the blood coagulation cascade. It is a Ca 2+ - dependent
transglutaminase or transamidating enzyme which forms intermolecular
gamma-glutamyl-epsilon-lysine crosslinks between fibrin molecules
resulting in the mechanical stabilization of the fibrin clot and its resistance to
proteolysis. Factor XIIIa may also function to stabilize cell surface molecules
and membranes. These Ca 2+ -dependent trans-glutaminases with thiol active
centers are widespread in animal tissues and have been associated with cell
proliferation, embryonic development and growth through the proliferation of
mammary stroma and epithelial elements. Normal mammary stroma, like
most collagenous connective tissue contains resident populations of CD34
positive dendritic interstitial cells and scattered factor XIIIa positive
collagen-associated dendrophages. Factor XIIIa has been examined to
determine its expression in normal and inflamed skin. Factor XIIIa positive
cells in human skin represent a specific population of bone marrow dermal
dendritic cells, distinct from Langerhans cells which share some features
common to mononuclear phagocytes. In benign skin conditions such as
inflammatory dermatoses eg atopic eczema and psoriasis, an increased
number of factor XIIIa positive cells in the upper dermis, closely associated
with lymphocytes, has been described.
Novocastra Fas (CD95)
Clone GM30
1 mL lyophilized NCL-FAS-310 F P (HIER)
Fas is a 48 kD transmembrane glycoprotein. It is a member of the nerve
growth factor receptor/tumor necrosis factor superfamily. This cell surface
molecule mediates receptor-triggered apoptosis (programmed cell death).
During embryonic and postembryonic development, many cells die by
means of apoptosis. This plays a major role in determining morphological
and functional maturity in a variety of systems, including the formation of the
neural network and clonal deletion of autoreactive T cells. Apoptosis is
accompanied by condensation of the cytoplasm, loss of plasma membrane
microvilli and extensive degradation of chromosomal DNA into oligomers of
about 180 base pairs. The Fas antigen is reported to be expressed on the
surface of various cell types, including activated T and B lymphocytes and
T lymphoblastoid cell lines.
Human small intestine: immunohistochemical staining for Fas antigen (CD95) using
NCL-FAS-310. Note membrane staining of absorptive epithelial cells. Paraffin section.
IVD
RUO*
Novocastra Fascin
Clone IM20
1 mL, 0.1 mL lyophilized NCL-FASCIN P (HIER) W
1 mL liquid NCL-L-FASCIN P (HIER) W RUO*
7 mL BOND ready-to-use PA0420 P (HIER) IVD
Human fascin is a 55 to 58 kD actin-bundling protein, whose actin binding
ability is regulated by phosphorylation. In normal tissues the detection of
fascin is reported to be predominantly restricted to dendritic cells and in the
thymus has been observed only in medullary dendritic cells. In reactive nodes,
interdigitating reticulum cells of T cell zones, cells in subcapsular areas, and
cells of the reticular network express fascin. Variable expression is seen in
follicular dendritic cells and endothelial cells. Lymphoid cells, myeloid cells
and plasma cells do not express fascin. However, in cases of Hodgkin's
disease, including nodular sclerosis, mixed cellularity lymphocyte depletion
and unclassified cases, most or all Reed Sternberg cells are reported to be
positive for fascin. Fascin expression may be induced by Epstein-Barr virus
(EBV) infection of B cells with the possibility that viral induction of fascin in
lymphoid or other cell types must also be considered in EBV-positive cases.
Novocastra Fas Ligand
Clone 5D1
1 mL lyophilized NCL-FAS-L P (HIER) W
Fas ligand, a cell surface molecule belonging to the tumor necrosis factor
family, binds to its receptor Fas, thus inducing apoptosis. Various cells
express Fas, whereas Fas ligand is reported to be expressed predominantly
on activated T cells. Fas and Fas ligand are involved in the downregulation
of immune reactions as well as T cell-mediated cytotoxicity. It is known that
tumor necrosis factor (TNF) works as a cachectin and mediates septic
shock, so like TNF, Fas ligand may work as an agent that causes tissue
damage. The Fas/Fas ligand system has been implicated both in maintaining
immune privilege and also as a key regulator in spermatogenesis.
Human prostate: immunohistochemical staining for Fas ligand using NCL-FAS-L. Note
membrane and cytoplasmic staining of glandular epithelial cells. Paraffin section.
Novocastra Feline Calicivirus (capsid
protein)
Clone 1G9
0.5 mL lyophilized NCL-1G9 W
RUO*
RUO*
The Caliciviridae are a family of positive-stranded RNA viruses of unique
morphology characterized by a series of cup-like depressions on the
surface of the virus. Feline Calicivirus (FCV) is a ubiquitous pathogen of cats
producing a variety of clinical symptoms, including oral ulceration, upper
respiratory tract infection and polyarthritis. FCV has a genome of 7.7kb
which encodes several proteins.
Product Specific Information
NCL-1G9 detects one of these, a capsid protein of 62 kD.
RUO*
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies