QF0159 Marketing Release Record

Primary Antibodies
Novocastra CD14
Clone 7
1 mL, 0.1 mL lyophilized NCL-CD14-223 P (HIER) W
1 mL liquid NCL-L-CD14-223 P (HIER) W IVD
Antigen Background
CD14 antigen is a glycosyl-phosphatidylinositol (GPI)-linked glycoprotein with
a molecular weight of 55 kD. The CD14 antigen is reported to be expressed on
cells of the myelomonocytic lineage including monocytes, macrophages and
Langerhans cells. Low expression is also reported on neutrophils and on B
cells. CD14 antigen is a receptor for bacterial lipopolysaccharide (LPS,
endotoxin) and the lipopolysaccharide binding protein (LBP). LBP and CD14
antigen serve two physiological roles. These proteins act as opsonin and
opsonic receptor, respectively, to promote the phagocytic uptake of bacteria
or LPS-coated particles by macrophages.
Human uterus: immunohistochemical staining for CD14 antigen using NCL-CD14-223. Note
membrane staining of macrophages within an endometrial gland and lymphocytes in the
stroma. Paraffin section.
Novocastra CD15
Clone BY87 Reference Range
1 mL, 0.1 mL lyophilized NCL-CD15 F P (HIER/Enzyme)
1 mL liquid NCL-L-CD15 F P (HIER/Enzyme) IVD
7 mL ready-to-use RTU-CD15 F P (HIER/Enzyme) IVD
Clone Carb-1
7 mL BOND ready-to-use PA0039 P (HIER)
Antigen Background
CD15 antigen, also known as X-hapten, is reported to be expressed on 90
percent of circulating human granulocytes, 30 to 60 percent of circulating
monocytes and is absent from normal lymphocytes. The CD15 antigen is also
expressed on Reed Sternberg cells of Hodgkin's disease and some
leukemias.
Product Specific Information
Please note that either enzyme pretreatment or heat induced epitope
retrieval (HIER) temperature antigen unmasking using 1mM EDTA (pH8.0)
solution may be used on fixed, paraffin-embedded tissue. The choice of
antigen unmasking technique to provide the best result should be determined
by the user.
/36
For detailed information on all products please visit our website:
www.leica-microsystems.com
IVD
RUO*
IVD
Hodgkin's disease: immunohistochemical staining for CD15 antigen using NCL-CD15. Note
membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg cells.
Paraffin section.
Novocastra CD16
Clone 2H7
1 mL, 0.1 mL lyophilized NCL-CD16 P (HIER)
CD16 antigen has a molecular weight of 50 to 70 kD and is a low affinity Fc
receptor for complexed IgG, Fc/gamma RIII, expressed on natural killer (NK)
cells, granulocytes, activated macrophages and a subset of T cells expressing
alpha-beta or gamma-delta T cell antigen receptors. The CD16 antigen
exists both as a glycosyl-phosphatidylinositol (GPI)-anchored protein in
polymorphonuclear cells and as a transmembrane protein in NK cells.
Human colon, ulcerative colitis: immunohistochemical staining for CD16 antigen using
NCL-CD16. Note intense membrane staining of infiltrating natural killer cells, granulocytes
and activated macrophages. Paraffin section.
Novocastra CD19
Clone BT51E Reference Range
1 mL, 0.1 mL liquid NCL-L-CD19-163 P (HIER) W
7 mL BOND ready-to-use PA0843 P (HIER) IVD
Clone 4G7/2E
1 mL, 0.1 mL lyophilized NCL-CD19-2 FC RUO*
Clone BT51E was developed to be effective on formalin-fixed, paraffinembedded
tissue sections.
Antigen Background
CD19 is a member of the immunoglobulin superfamily and has two Ig like
domains. It is a single chain glycoprotein present on the surface of B
lymphocytes and follicular dendritic cells of the hematopoietic system. CD19
is a crucial regulator in B cell development, activation and differentiation. On
B cells, CD19 associates with CD21, CD81 and CD225 (Leu-13) forming a signal
transduction complex. CD19 is expressed from the earliest recognizable B
cell lineage stage, through development to B cell differentiation but is lost on
maturation to plasma cells.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD
IVD