QF0159 Marketing Release Record

Primary Antibodies
Novocastra Epithelial Membrane
Antigen
Clone GP1.4
1 mL, 0.1 mL lyophilized NCL-EMA FP
1 mL liquid NCL-L-EMA FP IVD
7 mL ready-to-use RTU-EMA FP IVD
7 mL BOND ready-to-use PA0035 P (HIER)
Antigen Background
Epithelial membrane antigen (EMA), also known as episialin, is reported to be
expressed in a variety of normal and neoplastic epithelia. It has been reported
that markers to CD45 (LCA) when used in conjunction with markers to EMA
are useful in labelling cells of lymphoid origin whereas the combin-ation of
anti-cytokeratin antibodies together with EMA is useful to characterize cells
of epithelial origin. EMA is also notably described to be expressed in a subset
of Hodgkin’s lymphomas.
Novocastra Epithelial-Related Antigen
Clone MOC-31
1 mL lyophilized NCL-MOC-31 ASR
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra Epithelial Specific Antigen
Clone VU-1D9 Reference Range
1 mL, 0.1 mL lyophilized NCL-ESA F P (Enzyme) W
7 mL ready-to-use RTU-ESA F P (Enzyme) IVD
Antigen Background
Epithelial specific antigen (ESA) is a 40 kD cell surface glycoprotein. It is
reported to be expressed in the majority of human epithelial cells and is rarely
expressed in mesothelial cells.
Human appendix: immunohistochemical staining for epithelial specific antigen using NCL-ESA.
Note membrane staining of epithelial cells only. Paraffin section.
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IVD
IVD
IVD
Novocastra Epstein-Barr virus (early
antigens)
Clone G3-E31
1 mL lyophilized Epstein-Barr virus (early antigen diffuse)
NCL-EADE31 P (HIER) W O RUO*
Product Specific Information
NCL-EADE31 recognizes EBV early antigen diffuse (50 to 52 kD), encoded by
the BMRF-1 open reading frame. NCL-EARF2 recognizes EBV early antigen
restricted (85 kD). NCL-EADE31 and NCL-EARF2 react with formalin-fixed,
paraffin-embedded cells eg EBV-transformed B95-8 and RAJI cells using
heat induced epitope retrieval (HIER) with citrate buffer (pH6.0).
Epstein-Barr virus transformed B95-8 cells: immunocytochemical staining for early antigen
diffuse non-structural protein using NCL-EADE31. Note intense staining of infected cells only
where early virus replication is underway. Paraffin-embedded cells.
Novocastra Epstein-Barr virus-Induced
Gene 3 Protein
Clone EL8
1 mL lyophilized NCL-EBI-3 F P (HIER)
RUO*
Epstein-Barr virus (EBV)-associated Hodgkin's lymphoma (HL) and nasopharyngeal
carcinoma (NPC) usually occurs in individuals without clinically
apparent deficiencies in anti-viral immunity. Despite expressing viral
proteins, both tumors are apparently able to escape EBV-specific immunity
in vivo. EBI-3 is an EBV-induced cytokine homologous to the interleukin 12
p40 subunit which can heterodimerize with the interleukin 12 p35 subunit.
Researchers have suggested that EBI-3 protein may function to antagonize
interleukin 12 and to inhibit the development of a Th1 immune response. It
has been reported that EBI-3 protein is strongly expressed in Hodgkin's
Reed Sternberg (RS) cells in approximately 96 percent of HL cases,
independently of the EBV status of the tumor cells. EBI-3 protein has also
been reported to be detected in a small percentage of epithelial tumor cells
of NPC biopsies but not in Burkitt's lymphomas. EBI-3 protein may be an
additional component of the repertoire employed by Hodgkin's RS cells to
inhibit and effect anti-tumor or anti-viral immune response. EBI-3 protein
expression has also been reported in spleen, tonsil, mature dendritic cells,
colonic mucosa and at high levels in full term placenta.
Hodgkin's lymphoma: immunohistochemical staining for Epstein-Barr virus-induced gene 3
protein using NCL-EBI-3. Note cytoplasmic staining of infected cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.