QF0159 Marketing Release Record

Novocastra Peripherin
Clone PJM50
1 mL lyophilized NCL-PERIPH F P (HIER) W
Peripherin is a 57 kD type III intermediate filament protein that is expressed
in peripheral neurons, including enteric ganglion cells. Peripherin is
expressed in the developing peripheral nervous system and is highly
enriched in neuronal derivatives of the neural crest. The expression or
absence of peripherin may be used to demonstrate abnormalities of the
enteric nervous system. The assessment of the density of ganglion cells is of
importance in Hirschsprung's disease (HD)-related disorders. Peripherin is
also reported to be expressed in neural crest derived tumors such as
neuroblastomas and ganglioneuroblastomas.
Human small bowel: immunohistochemical staining for peripherin using NCL-PERIPH.
Note intense cytoplasmic staining of enteric ganglion cells and neural elements.
Paraffin section.
Novocastra PETA-3 (CD151)
Clone RLM30
1 mL lyophilized NCL-CD151 P (HIER)
See also CD151 (PETA-3) on page 46.
Novocastra P-glycoprotein (CD243)
Clone 5B12
1 mL lyophilized NCL-PGLYm F P (HIER)
Antigen Background
RUO*
The resistance of tumor cells to cytotoxic chemotherapeutic drugs is a major
problem in the treatment of cancer. Studies have linked the presence of a 170
to 180 kD cell membrane protein, P-glycoprotein, with resistance to a wide
range of lipophilic chemotherapeutic drugs, a phenomenon known as
multidrug resistance. P-glycoprotein is reported to be expressed in transporting
epithelia of several normal tissues, including liver, kidney, colon,
adrenal and brain.
IVD
RUO*
Novocastra Placental Alkaline
Phosphatase
Clone 8A9
1 mL, 0.1 mL lyophilized NCL-PLAP-8A9 F P (HIER)
1 mL liquid NCL-L-PLAP-8A9 F P (HIER) IVD
7 mL ready-to-use RTU-PLAP-8A9 F P (HIER) IVD
7 mL BOND ready-to-use PA0161 P (HIER) IVD
Antigen Background
Placental alkaline phosphatase (PLAP) is a membrane-associated
sialoglycoprotein enzyme normally present at high concentration in syncytiotrophoblasts
within the placenta during the third trimester of gestation.
The expression of PLAP was originally thought to be restricted to term
placenta but a human PLAP-like variant has been described which shares
more than 85 percent homology with PLAP itself. This high degree of
homology between PLAP and PLAP-like enzyme together with cross-reacting
antibodies has led to some confusion of the distribution of PLAP and PLAPlike
enzyme in various tissues. PLAP is reported to be expressed only in
normal term placenta, endocervix and fallopian tube and also in ovarian and
proximal gastrointestinal tumors. PLAP expression is rare in malignant germ
cell tumors. PLAP-like enzyme is reported to be predominantly found in
normal fetal and neonatal testis, and in thymus. It is also commonly expressed
in germ cell tumors and more recently described in seminomas.
Product Specific Information
Reports indicate that clone 8A9 stains seminomas and placenta indicating a
specificity for both PLAP and PLAP-like enzyme.
Human seminoma: immunohistochemical staining for placental alkaline phosphatase using
NCL-PLAP-8A9. Note intense membrane staining of tumor cells. Paraffin section.
Novocastra Plasma Cell Marker
Clone LIV3G11
1 mL lyophilized NCL-PC P (Enzyme)
RUO*
The plasma cell is the resultant terminal stage of B cell differentiation and
apart from morphological features may be distinguished from other B cells
by their lack of surface HLA class I and class II antigens, surface
immunoglobulin, Fc and C3 receptors or presence of intracytoplasmic
immunoglobulin. There have been some interesting studies where non-
Hodgkin’s lymphomas have been differentiated from plasmacytomas and
myelomas based on plasma cell markers (Seegmiller et al., American
Journal of Clinical Pathology. 127(2):172-174 (2007)).
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
/95
Primary Antibodies